Anti-Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)

  Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)

ICC/IF image of ab51317 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)

ab51317 staining Sca1/Ly6A/E in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

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