Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker (ab51317)
Key features and details
- Rat monoclonal [E13 161-7] to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker
See all Ly-6A/E (Sca-1) primary antibodies -
Description
Rat monoclonal [E13 161-7] to Ly-6A/E (Sca-1) - Hematopoietic Stem Cell Marker -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Mouse TH2 cell line. Flow Cyt: C57 BL/6 mouse splenocytes
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
E13 161-7 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.2µg for 106 cells.
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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Notes |
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Flow Cyt
Use 0.2µg for 106 cells. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
T-cell activation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsO-glycosylated. Not N-glycosylated.
Not phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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Alternative names
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
Images
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ab51317 stained mouse TH2 cells. The cells were 4% formaldehyde fixed for 4 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Flow cytometry staining of C57 BL/6 mouse splenocytes with ab51317 (right) or Rat IgG2aκ (ab18450) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % mouse serum/10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab51317) or Rat IgG2aκ (ab18450) isotype (1x 106 in 100μl at 0.2μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live lymphocytes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab51317 has been referenced in 32 publications.
- Figeac F et al. Impaired Bone Fracture Healing in Type 2 Diabetes Is Caused by Defective Functions of Skeletal Progenitor Cells. Stem Cells 40:149-164 (2022). PubMed: 35257177
- Zhou X et al. SM22α-lineage niche cells regulate intramembranous bone regeneration via PDGFRβ-triggered hydrogen sulfide production. Cell Rep 39:110750 (2022). PubMed: 35508129
- Sun W et al. SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression. Cell Commun Signal 20:104 (2022). PubMed: 35836260
- Guo S et al. GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity. Theranostics 11:8379-8395 (2021). PubMed: 34373748
- Ford MJ et al. Oviduct epithelial cells constitute two developmentally distinct lineages that are spatially separated along the distal-proximal axis. Cell Rep 36:109677 (2021). PubMed: 34496237