Anti-Schistosoma mansoni IgG Human ELISA Kit (ab108769)

Overview

  • Product nameAnti-Schistosoma mansoni IgG Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 24 5.1%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Schistosoma mansoni IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma.

     

    A 96-well plate has been precoated with Schistosoma mansoni antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidise (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Schistosoma mansoni-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Schistosoma mansoni IgG sample captured in plate.

  • Tested applicationsIndirect ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    IgG Sample Diluent Yellow, White cap 1 x 100ml
    Protein A HRP Conjugate Blue, Black cap 1 x 20ml
    Schistosoma mansoni (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Schistosoma mansoni IgG Cut-off Control Yellow, Green cap 1 x 3ml
    Schistosoma mansoni IgG Negative Control Yellow, Blue cap 1 x 2ml
    Schistosoma mansoni IgG Positive Control Yellow, Red cap 1 x 2ml
    Stop Solution Red cap 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research Areas
  • Alternative names
    • S. mansoni IgG

Applications

Our Abpromise guarantee covers the use of ab108769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References for Anti-Schistosoma mansoni IgG Human ELISA Kit (ab108769)

ab108769 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"