![Immunocytochemistry/ Immunofluorescence - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122) image](#ab77122if2.gif)
![Western blot - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122) image](#ab77122wb copy.gif)
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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Scramblase 1 antibody [13A6 [TEC-23]]
See all Scramblase 1 products (5) ...
Description
Mouse monoclonal [13A6 [TEC-23]] to Scramblase 1
Tested applications
WB, IP, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Rat
Immunogen
Pooled lipid rafts isolated from RBL-2H3 cells.
Positive control
Rat basophilic leukemia (RBL) cells and cell lysate. RBL lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
13A6 [TEC-23]
Isotype
IgG2a
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
Tissue specificity
Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
Sequence similarities
Belongs to the phospholipid scramblase family.
Post-translational
modifications
Known to be palmitoylated at one, yet undefined, site.
Cellular localization
Membrane.
Target information above from: UniProt accessionO15162
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ca(2+) dependent phospholipid scramblase 1 antibody
- Ca(2+)-dependent phospholipid scramblase 1 antibody
- Erythrocyte phospholipid scramblase antibody
see all
Database links
Anti-Scramblase 1 antibody [13A6 [TEC-23]] images:
Immunocytochemistry/ Immunofluorescence - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)
![Immunocytochemistry/ Immunofluorescence - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)](/ps/datasheet/Images/77/ab77122/ab77122if2.gif)
ab77122 staining Scramblase 1 in rat basophilic leukemia (RBL) cell line by Immunofluorescence.
Western blot - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)
![Western blot - Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)](/ps/datasheet/Images/77/ab77122/ab77122wb copy.gif)
All lanes : Anti-Scramblase 1 antibody [13A6 [TEC-23]] (ab77122) at 1 µg/ml
Lane 1 : Rat basophilic leukemia (RBL) cell line lysate
Lane 2 : Scramblase 1 immunoprecipitate from RBL lysate
Predicted band size : 35 kDa
Observed band size : 35 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)
This product has been referenced in:
- Smrz Det al. Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem 283:10904-18 (2008).Read more (PubMed: 18281686) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"