Anti-Seasonal and Swine H1N1 Neuraminidase antibody (ab91645)
Key features and details
- Rabbit polyclonal to Seasonal and Swine H1N1 Neuraminidase
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-Seasonal and Swine H1N1 Neuraminidase antibody -
Description
Rabbit polyclonal to Seasonal and Swine H1N1 Neuraminidase -
Host species
Rabbit -
Specificity
Recognizes the Neuraminidase from the both the Seasonal H1N1 Neuraminidase and Swine H1N1 Neuraminidase (A/California/14/2009 (H1N1)) in ELISA and WB. It will detect 2 ng of free peptide at 1 μg/mL.
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Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Influenza A virus (A/Georgia/20/2006(H1N1)), Influenza A virus (A/California/14/2009(H1N1)) -
Immunogen
Synthetic peptides from the Seasonal H1N1 Neuraminidase protein and the novel Swine H1N1 Neuraminidase protein (Genbank accession ACA33620 and ACQ76308).
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Positive control
- ELISA: Seasonal and Swine H1N1 Neuraminidase peptides, Swine H1N1 Neuraminidase recombinant protein. WB: Swine H1N1 Neuraminidase recombinant protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | ||
WB |
Notes |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible neuraminidase (NA) surface proteins. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited. -
Cellular localization
Cell Membrane -
Alternative names
- flu N1 antibody
- Influenza A (H1N1) Neuraminidase antibody
- Influenza N1 antibody
- NA antibody
Images
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All lanes : Anti-Seasonal and Swine H1N1 Neuraminidase antibody (ab91645) at 2 µg/ml
Lane 1 : 50 ng of Swine H1N1 Neuraminidase recombinant protein
Lane 2 : 100 ng of Swine H1N1 Neuraminidase recombinant protein
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution1h incubation at RT in 5% NFDM/TBST.
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Validation with H1N1 NA Protein Coating Antigen: Swine H1N1 Neuraminidase recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies: ab91645, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1/10000 dilution, incubated at RT for 1 hr.
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ELISA using ab91645 at 1 µg/ml, detects 2 ng of free peptide (Seasonal and Swine H1N1 Neuraminidase).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91645 has not yet been referenced specifically in any publications.