Anti-Sec24C antibody (ab122633)
Key features and details
- Rabbit polyclonal to Sec24C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sec24C antibody
See all Sec24C primary antibodies -
Description
Rabbit polyclonal to Sec24C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RLPSMTGPLL PGQSFGGPSV SQPNHVSSPP QALPPGTQMT GPLGPLPPMH SPQQPGYQPQ QNGSFGPARG PQSNYGGPYP AAPTF, corresponding to internal sequence amino acids 222-306 of Human Sec24C.
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Positive control
- U 251 MG cells; RT 4, U 251 MG, Human liver and tonsil lysates; Human stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the SEC23/SEC24 family. SEC24 subfamily. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 9632 Human
- Omim: 607185 Human
- SwissProt: P53992 Human
- Unigene: 81964 Human
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Alternative names
- Protein transport protein Sec24C antibody
- SC 24C antibody
- SC24 C antibody
see all
Images
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All lanes : Anti-Sec24C antibody (ab122633) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
ab122633 at 1/200 dilution staining Sec24C in paraffin-embedded Human stomach tissue by Immunohistochemistry.
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ab122633 at 4 µg/ml staining SEC24C in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab122633 has been referenced in 4 publications.
- Wei G et al. Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection. Nat Commun 13:5879 (2022). PubMed: 36202818
- Tang X et al. Export of polybasic motif-containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4. J Biol Chem 298:102687 (2022). PubMed: 36370847
- Rebensburg SV et al. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol 6:435-444 (2021). PubMed: 33649557
- Wang B et al. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest 128:3319-3332 (2018). PubMed: 29939162