Anti-Secretory Amyloid Precursor Protein antibody [OM84] (ab78011)

Overview

  • Product nameAnti-Secretory Amyloid Precursor Protein antibody [OM84]See all Secretory Amyloid Precursor Protein primary antibodies ...
  • Description
    Mouse monoclonal [OM84] to Secretory Amyloid Precursor Protein
  • Specificityab78011 reacts with Secretory Amyloid Precursor Protein (sAPP), but not with Amyloid-Beta protein 1-40.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Secretory Amyloid Precursor Protein secreted by the EJ-1 bladder carcinoma cell line.

  • EpitopeSecretory
  • Positive control
    • Brain

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.15M Sodium chloride, 10mM Sodium phosphate buffer, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberOM84
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab78011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 10 µg/ml. Predicted molecular weight of unprocessed precursor: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceAmyloid Precursor Protein (APP) functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. Immature APP (N glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha secretase cleavage, Secretory Amyloid Precursor Protein (sAPP) is released into the extracellular space and the C terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. APP is expressed in the brain, kidney, heart and spleen of fetal tissues; it is induced during neuronal differentiation. In adult brain, highest expression of APP is found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD).
    • Cellular localizationCell Membrane, Golgi Apparatus and Secreted
    • Database links
    • Alternative names
      • A4 antibody
      • AAA antibody
      • ABETA antibody
      • ABPP antibody
      • AD1 antibody
      • Alzheimer disease amyloid protein antibody
      • Alzheimer disease antibody
      • Amyloid beta A4 protein antibody
      • Amyloid beta A4 protein precursor antibody
      • Amyloid beta precursor protein antibody
      • APP antibody
      • APPI antibody
      • Beta amyloid peptide antibody
      • Cerebral vascular amyloid peptide antibody
      • CTFgamma antibody
      • CVAP antibody
      • Human mRNA for amyloid A4 precursor of Alzheimer's disease antibody
      • Peptidase nexin II antibody
      • PN II antibody
      • PN2 antibody
      • PreA4 antibody
      • Protease nexin II antibody
      see all

    References for Anti-Secretory Amyloid Precursor Protein antibody [OM84] (ab78011)

    ab78011 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    Our source for the antibody that we discussed (ab78011) as a possible replacement for the apparently defective APP antibody (ab82972) was not able fo find any references for it regarding the production or charact...

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    Thank you for your inquiry. Unfortunately, we do not have any antibodies against aa 628 - 770 of the sAPP available in our catalog. - The sAPP antibody ab82972 is directed against aa 540 - 589. - Unfortunately the epitope of the mon...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"