Anti-Secretory Amyloid Precursor Protein antibody (ab82972)

Overview

• Product nameAnti-Secretory Amyloid Precursor Protein antibodySee all Secretory Amyloid Precursor Protein primary antibodies ...
• Description
  Rabbit polyclonal to Secretory Amyloid Precursor Protein
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee
• Immunogen

  Synthetic peptide corresponding to a region within internal sequence amino acids 540 - 589 (RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDVLAN MISEPRISYG) of human Secretory Amyloid Precursor Protein (NP_000475).

  Run BLAST with Run BLAST with
• Positive controlMCF7 cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab82972 is purified by a peptide affinity chromatography method.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Amyloid

Applications

Target

• RelevanceAmyloid Precursor Protein (APP) functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. Immature APP (N glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha secretase cleavage, Secretory Amyloid Precursor Protein (sAPP) is released into the extracellular space and the C terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. APP is expressed in the brain, kidney, heart and spleen of fetal tissues; it is induced during neuronal differentiation. In adult brain, highest expression of APP is found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD).
• Cellular localizationCell Membrane, Golgi Apparatus and Secreted
• Database links
  • Entrez Gene: 351 Human
  • Entrez Gene: 11820 Mouse
  • Entrez Gene: 54226 Rat
  • Omim: 104760 Human
  • SwissProt: P05067 Human
  • SwissProt: P12023 Mouse
  • SwissProt: P08592 Rat

• Alternative names
  A4 antibodyAAA antibodyABETA antibody

  ABPP antibodyAD1 antibodyAlzheimer disease amyloid protein antibodyAlzheimer disease antibodyAmyloid beta A4 protein antibodyAmyloid beta A4 protein precursor antibodyAmyloid beta precursor protein antibodyAPP antibodyAPPI antibodyBeta amyloid peptide antibodyCerebral vascular amyloid peptide antibodyCTFgamma antibodyCVAP antibodyHuman mRNA for amyloid A4 precursor of Alzheimer's disease antibodyPeptidase nexin II antibodyPN II antibodyPN2 antibodyPreA4 antibodyProtease nexin II antibody

  see all

Anti-Secretory Amyloid Precursor Protein antibody images

• 

  Western blot - Secretory Amyloid Precursor Protein antibody (ab82972)
  Anti-Secretory Amyloid Precursor Protein antibody (ab82972) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 87 kDa
  Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.