Anti-Secretory Amyloid Precursor Protein antibody (ab82972)

Overview

  • Product nameAnti-Secretory Amyloid Precursor Protein antibody
    See all Secretory Amyloid Precursor Protein primary antibodies
  • Description
    Rabbit polyclonal to Secretory Amyloid Precursor Protein
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540 - 589 (RMNQSLSLLY NVPAVAEEIQ DEVDELLQKE QNYSDDVLAN MISEPRISYG) of human Secretory Amyloid Precursor Protein (NP_000475).

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab82972 is purified by a peptide affinity chromatography method.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab82972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 87 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/1562500.

Target

  • RelevanceAmyloid Precursor Protein (APP) functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. Immature APP (N glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha secretase cleavage, Secretory Amyloid Precursor Protein (sAPP) is released into the extracellular space and the C terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. APP is expressed in the brain, kidney, heart and spleen of fetal tissues; it is induced during neuronal differentiation. In adult brain, highest expression of APP is found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD).
  • Cellular localizationCell Membrane, Golgi Apparatus and Secreted
  • Database links
  • Alternative names
    • A4 antibody
    • AAA antibody
    • ABETA antibody
    • ABPP antibody
    • AD1 antibody
    • Alzheimer disease amyloid protein antibody
    • Alzheimer disease antibody
    • Amyloid beta A4 protein antibody
    • Amyloid beta A4 protein precursor antibody
    • Amyloid beta precursor protein antibody
    • APP antibody
    • APPI antibody
    • Beta amyloid peptide antibody
    • Cerebral vascular amyloid peptide antibody
    • CTFgamma antibody
    • CVAP antibody
    • Human mRNA for amyloid A4 precursor of Alzheimer's disease antibody
    • Peptidase nexin II antibody
    • PN II antibody
    • PN2 antibody
    • PreA4 antibody
    • Protease nexin II antibody
    see all

Anti-Secretory Amyloid Precursor Protein antibody images

  • Anti-Secretory Amyloid Precursor Protein antibody (ab82972) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 87 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Secretory Amyloid Precursor Protein antibody (ab82972)

ab82972 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. Unfortunately, we do not have any antibodies against aa 628 - 770 of the sAPP available in our catalog. - The sAPP antibody ab82972 is directed against aa 540 - 589. - Unfortunately the epitope of the mon...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"