Anti-Selenophosphate synthetase 1 antibody (ab96542)
Key features and details
- Rabbit polyclonal to Selenophosphate synthetase 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Selenophosphate synthetase 1 antibody
See all Selenophosphate synthetase 1 primary antibodies -
Description
Rabbit polyclonal to Selenophosphate synthetase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-174 of Human Selenophosphate synthetase 1.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Synthesizes selenophosphate from selenide and ATP. -
Tissue specificity
Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased. Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. -
Sequence similarities
Belongs to the selenophosphate synthase 1 family. Class II subfamily. -
Cellular localization
Cytoplasm and Cell membrane. Nucleus membrane. - Information by UniProt
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Database links
- Entrez Gene: 507470 Cow
- Entrez Gene: 22929 Human
- Entrez Gene: 109079 Mouse
- Entrez Gene: 291314 Rat
- Entrez Gene: 324947 Zebrafish
- Omim: 600902 Human
- SwissProt: Q0VC82 Cow
- SwissProt: P49903 Human
see all -
Alternative names
- MGC4980 antibody
- SELD antibody
- Selenide antibody
see all
Images
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ab96542 at 1/200 dilution staining Selenophosphate synthetase 1 in paraformaldehyde fixed HeLa cells. Lower image is merged with DNA probe.
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All lanes : Anti-Selenophosphate synthetase 1 antibody (ab96542) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96542 has been referenced in 1 publication.
- Akieda Y et al. Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo. Nat Commun 10:4710 (2019). PubMed: 31624259