|
|
|
|
Western blot - Semaphorin 3A antibody (ab23393)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Semaphorin 3A antibody(ab23393)
(enlarge)
|
|
Western blot - Semaphorin 3A antibody (ab23393)
(enlarge)
|
|
|
|
|
Product Name
|
Semaphorin 3A antibody
|
| |
See all Semaphorin 3A antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Semaphorin 3A
|
|
Immunogen
|
Synthetic peptide (coupled to carrier protein): RDGPNYQWVPYQGR, corresponding to amino acids 359-372 of Human Semaphorin 3A.
BLAST 'RDGPNYQWVPYQGR' with  or 
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Chk, JRfsh, Xl, Zfsh
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/2000.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution (PMID 19443185).
IHC-FoFr: Use at an assay dependent dilution (PMID 18574596).
WB: 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Rat brain lysate for SDS PAGE immunoblots.
|
|
Cellular localization
|
Plasma membrane and Secreted
|
|
Research areas
|
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
|
|
Relevance
|
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
This antibody was affinity purified.
|
|
Form
|
Liquid
|
|
Concentration
|
0.400 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Semaphorin 3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Semaphorin 3A antibody images, references, products related to ab23393 and other tools.
|
| |
Semaphorin 3A antibody images:
|
 Western blot - Semaphorin 3A antibody (ab23393)
Lanes 1 - 2 : Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3 - 4 : Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lane 2 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Lane 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Predicted band size : 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Semaphorin 3A antibody(ab23393)
Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
Western blot - Semaphorin 3A antibody (ab23393)
Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 106 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute This image is a courtesy of Anonimous Abreview
See Abreview
|
| |
References for Semaphorin 3A antibody (ab23393)
|
|
This product has been used in: (two most recent references)
Tominaga M et al. Psoralen-ultraviolet A therapy alters epidermal Sema3A and NGF levels and modulates epidermal innervation in atopic dermatitis. J Dermatol Sci 55:40-6 (2009). IHC-Fr; Human. PubMed: 19443185
Tatsumi R et al. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation. Am J Physiol Cell Physiol 297:C238-52 (2009). PubMed: 19515904
See all 5 publication references for this product.
If you publish research using ab23393 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Semaphorin 3A
|
| |
Semaphorin 3A antibody - more information
|
Customer reviews (feedback) regarding Semaphorin 3A antibody |
Customer FAQs regarding Semaphorin 3A antibody |
Protocols for Semaphorin 3A antibody |
Price and availability of products related to Semaphorin 3A antibody |
| |
Semaphorin 3A antibody - related products:
|
|
|
| |
Semaphorin 3A antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Semaphorin 3A antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
