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Products:Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Overview
Product name
Anti-Semaphorin 3A antibody
See all Semaphorin 3A products (7) ...
Description
Rabbit polyclonal to Semaphorin 3A
Tested applications
IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
Immunogen
Synthetic peptide (coupled to carrier protein): RDGPNYQWVPYQGR, corresponding to amino acids 359-372 of Human Semaphorin 3A.
RDGPNYQWVP YQGR
Positive control
Rat brain lysate for SDS PAGE immunoblots.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide.
Constituents: 50% Glycerol, PBS, 1mg/ml BSA.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr
ELISA
Application notes
ELISA: 1/2000.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution (PMID 19443185).
IHC-FoFr: Use at an assay dependent dilution (PMID 18574596).
WB: 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Cellular localization
Plasma membrane and Secreted
Alternative names
- Coll 1 antibody
- Coll1 antibody
- Hsema I antibody
see all
Database links
- Entrez Gene: 395825 Chicken
- Entrez Gene: 10371 Human
- Entrez Gene: 20346 Mouse
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q90607 Chicken
- SwissProt: Q14563 Human
- SwissProt: O08665 Mouse
see all
Anti-Semaphorin 3A antibody images:
Western blot - Semaphorin 3A antibody (ab23393)
Lanes 1 - 2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3 - 4 : Anti-Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lane 2 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Lane 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Predicted band size : 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Semaphorin 3A antibody(ab23393)
Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Semaphorin 3A antibody (ab23393)
Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 106 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This image is a courtesy of Anonymous Abreview
Immunohistochemistry (Frozen sections) - Anti-Semaphorin 3A antibody (ab23393)
ab23393 staining Semaphorin 3A in 16 µm thick sections of Apteronotus leptorhynchus brain, spinal cord by Immunohistochemistry (Frozen sections).Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab23393 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.
Image courtesy of Dr Ruxandra Sirbulescu by Abreview.
References for Anti-Semaphorin 3A antibody (ab23393)
This product has been referenced in:
- Atkinson-Leadbeater Ket al. Dynamic expression of axon guidance cues required for optic tract development is controlled by fibroblast growth factor signaling. J Neurosci 30:685-93 (2010).Read more (PubMed: 20071533) »
- Tominaga Met al. Psoralen-ultraviolet A therapy alters epidermal Sema3A and NGF levels and modulates epidermal innervation in atopic dermatitis. J Dermatol Sci 55:40-6 (2009). IHC-Fr; Human.Read more (PubMed: 19443185) »
See all 6 publications for this product
Publishing research using ab23393? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"