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Western blot - Semaphorin 3A antibody (ab25999)
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Product Name
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Semaphorin 3A antibody
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See all Semaphorin 3A antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Semaphorin 3A
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Immunogen
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Synthetic peptide (Human)(Peptide available as ab42770.)
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Reacts with
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Hu
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Does not react with
(species key)
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Rat
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Specificity
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ab25999 recognises Semaphorin 3A.
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/2000.
WB: 1/1000. Predicted molecular weight: 89 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Plasma membrane and Secreted
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Research areas
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Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
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Relevance
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One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1.0mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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This antibody may be unstable at +4°C. We suggest that it is stored at -20°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Semaphorin 3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Semaphorin 3A antibody images, references, products related to ab25999 and other tools.
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Semaphorin 3A antibody images:
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 Western blot - Semaphorin 3A antibody (ab25999)
Lanes 1 - 2 : Semaphorin 3A antibody (ab23393)
Lanes 3 - 4 : Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lane 2 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Lane 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Predicted band size : 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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References for Semaphorin 3A antibody (ab25999)
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This product has been used in:
Esselens C et al. The cleavage of semaphorin 3C induced by ADAMTS1 promotes cell migration. J Biol Chem 285:2463-73 (2010). PubMed: 19915008
If you publish research using ab25999 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Semaphorin 3A
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Semaphorin 3A antibody - more information
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Semaphorin 3A antibody - related products:
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