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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab63706? Please let us know so that we can cite the reference in this datasheet
ab63706 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Semaphorin 3A antibody (ab63706)
All lanes : Anti-Semaphorin 3A antibody (ab63706) at 2 µg/ml
Lane 1 :
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Semaphorin 3A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Semaphorin 3A antibody for IHC-P in Mouse (63706)
Semaphorin 3A antibody for IHC-P in Rat (63706)
Semaphorin 3A antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (63706)
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