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Products:Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Overview
Product name
Anti-Semaphorin 3A antibody
See all Semaphorin 3A products (7) ...
Description
Rabbit polyclonal to Semaphorin 3A
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Semaphorin 3A.
(Peptide available as ab71920.)
Positive control
This antibody gave a positive signal in the following lysates: SHSY-5Y whole cell, SK N SH whole cell and day 0 mouse brain tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
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Western blot - Semaphorin 3A antibody (ab63706)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab63706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
Target
Relevance
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Cellular localization
Plasma membrane and Secreted
Alternative names
- Coll 1 antibody
- Coll1 antibody
- Hsema I antibody
see all
Database links
- Entrez Gene: 10371 Human
- Entrez Gene: 20346 Mouse
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q14563 Human
- SwissProt: O08665 Mouse
- SwissProt: Q5BL08 Mouse
- SwissProt: Q63548 Rat
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Anti-Semaphorin 3A antibody images:
Western blot - Semaphorin 3A antibody (ab63706)
All lanes : Anti-Semaphorin 3A antibody (ab63706) at 2 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Semaphorin 3A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Semaphorin 3A antibody (ab63706)
ab63706 has not yet been referenced specifically in any publications.
Publishing research using ab63706? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"