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Western blot - Semaphorin 3A antibody (ab63706)
(enlarge)
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Product Name
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Semaphorin 3A antibody
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See all Semaphorin 3A antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Semaphorin 3A
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Semaphorin 3A. (Peptide available as ab71920.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
SHSY-5Y whole cell, SK N SH whole cell and day 0 mouse brain tissue.
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Cellular localization
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Plasma membrane and Secreted
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Research areas
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Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
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Relevance
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One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Semaphorin 3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Semaphorin 3A antibody images, references, products related to ab63706 and other tools.
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Semaphorin 3A antibody images:
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 Western blot - Semaphorin 3A antibody (ab63706)
All lanes : Semaphorin 3A antibody (ab63706) at 2 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Semaphorin 3A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to Semaphorin 3A
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Semaphorin 3A antibody - more information
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Customer reviews (feedback) regarding Semaphorin 3A antibody |
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Semaphorin 3A antibody - related products:
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Semaphorin 3A antibody - other tools:
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