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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab80011? Please let us know so that we can cite the reference in this datasheet
ab80011 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Semaphorin 3A antibody (ab80011)
ab80011, at a 1/10 dilution, staining Semaphorin 3A in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Peroxidase conjugated secondary antibody was used, followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Semaphorin 3A antibody (ab80011)
ICC/IF image of ab80011 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80011, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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