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Products:Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Overview
Product name
Anti-Semaphorin 3A antibody
See all Semaphorin 3A products (7) ...
Description
Rabbit polyclonal to Semaphorin 3A
Tested applications
ICC/IF, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH conjugated synthetic peptide selected from the center region of Human Semaphorin 3A.
Positive control
Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.
Target
Relevance
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
Cellular localization
Plasma membrane and Secreted
Alternative names
- Coll 1 antibody
- Coll1 antibody
- Hsema I antibody
see all
Database links
- Entrez Gene: 10371 Human
- Omim: 603961 Human
- SwissProt: Q14563 Human
- Unigene: 252451 Human
Anti-Semaphorin 3A antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Semaphorin 3A antibody (ab80011)
ab80011, at a 1/10 dilution, staining Semaphorin 3A in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Peroxidase conjugated secondary antibody was used, followed by DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Semaphorin 3A antibody (ab80011)
ICC/IF image of ab80011 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80011, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Semaphorin 3A antibody (ab80011)
ab80011 has not yet been referenced specifically in any publications.
Publishing research using ab80011? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"