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Product Name
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Semaphorin 3A peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide corresponding to amino acids in the central region of human Sema3A.
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Specificity
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The ab42768 peptide blocks antibodies that recognise amino acids in the central region of human Sema3A. This sequence is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.
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Tested applications
(see key)
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BL, ELISA
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Application notes
(see key)
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Recommended dilutions
BL: Use at a concentration of 1 µg/ml. Recommended for blocking antibody reactivity in Western Blot and Immunocytochemistry.
ELISA: 10-100ng/well, established in 96-well Nunc immunoplates where peptide was bound to plates for 2hrs in 0.1M sodium carbonate buffer, pH 8.5.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
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Relevance
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One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Semaphorin 3A peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Semaphorin 3A
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