Recombinant Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (ab180174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13458(B)] to SEMA3D - C-terminal
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SEMA3D antibody [EPR13458(B)] - C-terminal
See all SEMA3D primary antibodies -
Description
Rabbit monoclonal [EPR13458(B)] to SEMA3D - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7 cells, 293T, MCF7, fetal brain and fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13458(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 90 kDa. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Domain
Strong binding to neuropilin is mediated by the carboxy third of the protein. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 223117 Human
- Entrez Gene: 108151 Mouse
- Entrez Gene: 246262 Rat
- Omim: 609907 Human
- SwissProt: O95025 Human
- SwissProt: Q8BH34 Mouse
- Unigene: 201340 Human
- Unigene: 89313 Mouse
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Alternative names
- Coll 2 antibody
- Coll2 antibody
- Collapsin 2 antibody
see all
Images
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Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling SEMA3D using ab180174 at 1/10 (red) or a rabbit IgG (negative) (green).
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All lanes : Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (ab180174) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Fetal brain lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab180174 has not yet been referenced specifically in any publications.