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Overview

  • Product nameAnti-Separase antibody [2329C1a]See all Separase primary antibodies ...
  • Description
    Mouse monoclonal [2329C1a] to Separase
  • Tested applicationsWB, Dot Blot more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Tagged recombinant fragment: NPGLNGRSRR AKKVASRHCE ERRPQRASDQ ARPGPEIMRT IPEEELTDNW RKMSFEILRG SDGEDSASGG KTPAPGPEAA SGEWELLRLD SSKKKLPSPC PDKESDKD, corresponding to internal sequence amino acids 1448-1555 of Human Separase

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlImmunising recombinant Human Separase protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab84868 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • Clonality Monoclonal
  • Clone number2329C1a
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab84868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 233 kDa.
Dot Blot Dot: Use at an assay dependent dilution.

Target

  • FunctionCaspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • Sequence similaritiesBelongs to the peptidase C50 family.
  • Post-translational
    modifications    Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q14674 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Caspase like protein ESPL1 antibodyCaspase-like protein ESPL1 antibodyESP 1 antibody
        ESP1 antibodyESPL 1 antibodyESPL1 antibodyESPL1_HUMAN antibodyExtra spindle poles like 1 antibodyExtra spindle poles like 1 protein antibodyExtra spindle poles-like 1 protein antibodyFLJ46492 antibodyKIAA0165 antibodySeparase antibodySeparin antibodySimilar to fission yeast cut1and gene antibodySSE antibody
      see all

    Anti-Separase antibody [2329C1a] images

    References for Anti-Separase antibody [2329C1a] (ab84868)

    ab84868 has not yet been referenced specifically in any publications.

    Publishing research using ab84868 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"