Anti-Septin 11 antibody (ab183529)
Key features and details
- Rabbit polyclonal to Septin 11
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Septin 11 antibody
See all Septin 11 primary antibodies -
Description
Rabbit polyclonal to Septin 11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human Septin 11 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_060713.1.
Database link: Q9NVA2 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab183529 was affinity purified using an epitope specific to Septin 11 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy. -
Tissue specificity
Widely expressed, except in leukocytes. -
Involvement in disease
Note=A chromosomal aberration involving SEPT11 may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with MLL/HRX. -
Sequence similarities
Belongs to the septin family. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > synapse. Cell projection > dendritic spine. Cell projection > axon. Partly colocalizes with stress fibers and microtubules. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. - Information by UniProt
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Database links
- Entrez Gene: 461233 Chimpanzee
- Entrez Gene: 535746 Cow
- Entrez Gene: 101154542 Gorilla
- Entrez Gene: 100051283 Horse
- Entrez Gene: 55752 Human
- Entrez Gene: 52398 Mouse
- Entrez Gene: 305227 Rat
- Entrez Gene: 698867 Rhesus monkey
see all -
Alternative names
- SEP11_HUMAN antibody
- SEPT 11 antibody
- Sept11 antibody
see all
Images
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Detection of Septin 11 by Western Blot of Immunoprecipitate.
ab183529 at 0.2µg/ml labeling Septin 11 in HeLa whole cell lysate immunoprecipitated using ab183529 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. -
All lanes : Anti-Septin 11 antibody (ab183529) at 0.2 µg
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183529 has not yet been referenced specifically in any publications.