Recombinant Anti-Septin 8 antibody [EPR16099] (ab191404)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16099] to Septin 8
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Septin 8 antibody [EPR16099]
See all Septin 8 primary antibodies -
Description
Rabbit monoclonal [EPR16099] to Septin 8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HeLa, HCT-116, U-87 MG, C6, PC-12 and NIH/3T3 cell lysates; Human stomach and mouse brain tissues; K562 and C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16099 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/200.
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IP |
1/50.
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Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/200. |
IP
1/50. |
Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Septin 8 (SEPT8) is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. There are 3 isoforms produced by alternative splicing. -
Cellular localization
Cytoplasmic; cytoskeleton. -
Database links
- Entrez Gene: 23176 Human
- Entrez Gene: 20362 Mouse
- Entrez Gene: 303135 Rat
- Omim: 608418 Human
- SwissProt: Q92599 Human
- SwissProt: Q8CHH9 Mouse
- SwissProt: B0BNF1 Rat
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Alternative names
- KIAA0202 antibody
- OTTHUMP00000211755 antibody
- SEP 2 antibody
see all
Images
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All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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Immunohistochemical analysis of paraffin-embedded, Human stomach tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized C6 cells labeling Septin 8 with ab191404 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).
The two negative controls used anti-Septin 8 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Septin 8 with ab191404 at 1/150 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti-rabbit IgG (FITC) was used as secondary antibodyat 1/150 dilution.
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Western blot analysis of Septin 8 in K562 cell lysate immunoprecipitated using ab191404 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191404 has not yet been referenced specifically in any publications.