Anti-Serine Palmitoyltransferase antibody (ab23696)
- Product nameAnti-Serine Palmitoyltransferase antibodySee all Serine Palmitoyltransferase primary antibodies ...
- DescriptionRabbit polyclonal to Serine Palmitoyltransferase
- SpecificityThis antibody detects human serine palmitoyltransferase subunit SPT2
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis, Zebrafish, African Green Monkey
Synthetic peptide: LDRPFDETTYEETED, corresponding to amino acids 548-562 of Human Serine Palmitoyltransferase.
- Positive control
- Mouse brain homogenate, rat kidney supernatant, human liver microsome.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, 0.8% Sodium chloride, 0.3% Tris
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody was affinity purifed.
- Clonality Polyclonal
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab23696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.|
|IHC-P||IHC-P: Use a concentration of 1 µg/ml.|
- FunctionSerine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate.
- Tissue specificityWidely expressed.
- PathwayLipid metabolism; sphingolipid metabolism.
- Involvement in diseaseDefects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.
- Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
- Cellular localizationEndoplasmic reticulum membrane.
- KIAA0526 antibody
- LCB 2 antibody
- LCB2 antibody
- LCB2a antibody
- Long chain base biosynthesis protein 2 antibody
- Long chain base biosynthesis protein 2a antibody
- Serine palmitoyl CoA transferase 2 antibody
- Serine palmitoyltransferase 2 antibody
- Serine palmitoyltransferase long chain base subunit 2 antibody
- Serine palmitoyltransferase subunit II antibody
- Serine-palmitoyl-CoA transferase 2 antibody
- SPT 2 antibody
- SPT2 antibody
- SPTC2_HUMAN antibody
- SPTLC 2 antibody
- Sptlc2 antibody
Anti-Serine Palmitoyltransferase antibody images
All lanes : Anti-Serine Palmitoyltransferase antibody (ab23696) at 1 µg/ml
Lane 1 : 30ug mouse brain homogenate.
Lane 2 : 30ug rat kidney supernatant.
Lane 3 : 30ug human liver microsome.
Predicted band size : 62 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)
References for Anti-Serine Palmitoyltransferase antibody (ab23696)
This product has been referenced in:
- Erickson KA et al. AICAR inhibits ceramide biosynthesis in skeletal muscle. Diabetol Metab Syndr 4:45 (2012). WB ; Mouse . Read more (PubMed: 23134616) »
- Thrush AB et al. A single prior bout of exercise protects against palmitate-induced insulin resistance despite an increase in total ceramide content. Am J Physiol Regul Integr Comp Physiol 300:R1200-8 (2011). WB ; Rat . Read more (PubMed: 21325642) »