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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide.
Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Primary antibody notes Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).Can be blocked with
Serotonin N-acetyltransferase peptide (ab4975).
By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.
Function Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Tissue specificity Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
Pathway Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
Involvement in disease Delayed sleep phase syndrome
Sequence similarities Belongs to the acetyltransferase family. AANAT subfamily. Contains 1 N-acetyltransferase domain.
Post-translational modifications cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.
Cellular localization Cytoplasm.
Information by UniProt
Aralkylamine N-acetyltransferase antibody
Anti-Serotonin N-acetyltransferase (phospho T29) antibody images
Western blot - Serotonin N-acetyltransferase (phospho T29) antibody (ab3439)
Predicted band size : 23 kDa
Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.
References for Anti-Serotonin N-acetyltransferase (phospho T29) antibody (ab3439)
has not yet been referenced specifically in any publications.
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