Anti-Serotonin antibody [5HT-H209] (ab16007)

  Immunocytochemistry - Serotonin antibody [5HT-H209] (ab16007)

ab16007 mouse monoclonal [5HT-H209] used on cryopreserved ventral mesencephalic neurons dissected at embryonic day 14. Cells were thawed plated and grown for 14 days, fixed and stained with ab16007 at 1/50 overnight.

Image courtesy of QBM Cell Sciences, Ottawa, Canada

  Immunohistochemistry (Frozen sections) - Serotonin antibody [5HT-H209] (ab16007)

ab16007 staining mouse intestinal tissue sections by IHC-Fr.  The tissue was fixed with acetone, permeabilised by 0.1% TX-100 in AB-solution, and blocked with 10% serum for 30 minutes at 25°C.  The primary antibody was diluted 1/200 in 10% normal goat serum in PBS, 0.1% TX100 and incubated for 24 hours at 4°C.  A FITC-conjugated rabbit anti-mouse was used as the secondary. 

This image is courtesy of an Abreview submitted by Dr Andreas Gille

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serotonin antibody [5HT-H209] (ab16007)

Ab16007 staining of serotonin in human appendix by formalin-fixed and paraffin-embedded IHC. Note cytoplasmic staining of serotonin-secreting cells.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serotonin antibody [5HT-H209] (ab16007)

ab16007 staining Serotonin in pig small tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Citrate Buffer (pH 6) and then blocking with 10% serum was performed for 20 minutes at 22°C. The primary antibody was diluted 1/300 and incubated with sample in TBS/TX(0,3%)/BSA(1%) for 16 hours at 4°C. A Biotin conjugated goat polyclonal to mouse IgG was used at dilution at 1/200 as secondary antibody.

This image is a courtesy of Nele Verheyen

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