Serotonin antibody [5HT-H209] (ab16007)

Product Name 

Serotonin antibody [5HT-H209]

Product type 

Primary antibodies

Description 

Mouse monoclonal [5HT-H209] to Serotonin

Immunogen 

5 hydroxytryptamine hydrochloride.

Reacts with 
(species key)

Hu, Ms, Rat, Dog, Gpig, Pig

Tested applications 
(see key)

ICC, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/50.
IHC-P: 1/20 - 1/40. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Appendix.

Cellular localization 

Cytoplasmic and Secreted

Research areas 

Neuroscience >> Neurotransmitter >> Biogenic Amines >> Serotonin / 5HT

Relevance 

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

Ab16007 may be a useful tool for characterization of carcinoids.
In a study of 48 cases of adenomas and carcinoid tumours of the middle ear, the antibody labelled tumour cells in 12 cases (25%). In 1 case of carcinoid tumour of the middle ear, the antibody labelled tumour cells. In a study of 43 cases of bronchial carcinoid tumours, the antibody labelled tumour cells in 13 cases (30%). Cells labelled by the antibody display a cytoplasmic staining pattern.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

5HT-H209

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 0.05M Tris HCl, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

See below for Serotonin antibody [5HT-H209] images, references, products related to ab16007 and other tools.

Serotonin antibody [5HT-H209] images:

References for Serotonin antibody [5HT-H209] (ab16007)

  This product has been used in: (two most recent references)

Ootani A et al. Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med 15:701-6 (2009). IHC-P; Mouse. PubMed: 19398967

Torske KR & Thompson LD Adenoma versus carcinoid tumor of the middle ear: a study of 48 cases and review of the literature. Mod Pathol 15:543-55 (2002). PubMed: 12011260

See all 4 publication references for this product.


If you publish research using ab16007 please let us know so that we can cite the reference on this datasheet.

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