Serotonin antibody [YC5/45] (ab6336)

Product Name 

Serotonin antibody [YC5/45]

Product type 

Primary antibodies

Description 

Rat monoclonal [YC5/45] to Serotonin

Immunogen 

5HT-bovine seroalbumin.

Reacts with 
(species key)

Hu, Ms, Rat, Gpig, Rb, Xl

Specificity 

Detects Serotonin containing cell bodies and terminals in the central and peripheral nervous systems. Does not label catecholamine rich neurons in formaldehyde fixed tissue sections. The immunoreactivity is obliterated by agents which inhibit the biosynthesis of 5HT. Reacts with carcinoid tumours. Demonstrates cross reactivity with dopamine, tryptamine and methoxytryptamine in liquid media tests.

Tested applications 
(see key)

IHC-FoFr, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fo: 1/100- 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at a concentration of 1 - 5 µg/ml (see Ni et al) . Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Secreted

Research areas 

Neuroscience >> Neurotransmitter >> Biogenic Amines >> Serotonin / 5HT

Relevance 

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

YC5/45

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Serotonin antibody [YC5/45] images:

References for Serotonin antibody [YC5/45] (ab6336)

  This product has been used in: (two most recent references)

Marino P et al. A polysialic acid mimetic peptide promotes functional recovery in a mouse model of spinal cord injury. Exp Neurol 219:163-74 (2009). PubMed: 19445935

Hass N et al. A cluster of gustducin-expressing cells in the mouse stomach associated with two distinct populations of enteroendocrine cells. Histochem Cell Biol 128:457-71 (2007). IHC-FoFr; Mouse. PubMed: 17874119

See all 8 publication references for this product.


If you publish research using ab6336 please let us know so that we can cite the reference on this datasheet.

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