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Products:Neuroscience >> Neurotransmitter >> Biogenic Amines >> Serotonin / 5HT
Overview
Product name
Anti-Serotonin antibody
See all Serotonin products (9) ...
Description
Rabbit polyclonal to Serotonin
Tested applications
IHC-P, IHC-Frmore details
Immunogen
Serotonin coupled to bovine serum albumin with paraformaldehyde.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, Whole Serum
Purity
Whole antiserum
Purification notes
Delipidised.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Serotonin / 5HT
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Serotonin antibody (ab10385)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/100 - 1/200.IHC-P: 1/100 - 1/200.
IHC-Fr: 1/100 - 1/200.(Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant.)
Target
Relevance
Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
Cellular localization
Cytoplasmic and Secreted
Alternative names
- 5 HT antibody
- 5 hydroxytryptamine antibody
- 5HT antibody
Anti-Serotonin antibody images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - Serotonin antibody (ab10385)
IHC in brainstem using ab10385 at a 1/1000 dilution.
References for Anti-Serotonin antibody (ab10385)
This product has been referenced in:
- Rossi SLet al. Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord. PLoS One 5:e11852 (2010).Read more (PubMed: 20686613) »
- Yeung LYet al. Localization of 5-HT1A and 5-HT2A positive cells in the brainstems of control age-matched and Alzheimer individuals. Age (Dordr) 32:483-95 (2010).Read more (PubMed: 20508993) »
See all 3 publications for this product
Publishing research using ab10385? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"