Serotonin antibody (ab8882)

Product Name 

Serotonin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Serotonin

Immunogen 

5 hydroxytry ptamine-gl utaraldehy

Specificity 

Using a conjugate 5-hydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

(Compound = Cross-Reactivity Ratio §)

5-Hydroxytryptamine-G-PL = 1
5-Hydroxytryptamine=G=PL¥ = 1/10
5-Methoxytryptamine-G-PL = 1/25
Tryptamine-G-PL = 1/51
5-Hydroxytryptophan-G-PL = 1/175
5-Hydroxytryptamine = 1/1,150
Tryptophan-G-PL = 1/3,400
5-Methoxytryptophan-G-PL = 1/19,800

G = Glutaraldehyde,
PL = poly-Lysine
§ 5-Hydroxytryptamine-G-PL concentration/unconjugated or conjugated indolealkylamine concentration at halfdisplacement.
¥ Non-reduced conjugate

Tested applications 
(see key)

ELISA, ICC, IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
The recommended dilution can be between 1:2,000-1:5,000.

Cellular localization 

Cytoplasmic and Secreted

Research areas 

Neuroscience >> Neurotransmitter >> Biogenic Amines >> Serotonin / 5HT

Relevance 

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS, 0.01% Thimerosal

Material safety datasheet (MSDS) for this product:
Thimerosal MSDS

Form 

Liquid

Storage instructions 

Store at +4°C.

References for Serotonin antibody (ab8882)

  This product has been used in: (two most recent references)

Bakst MR & Akuffo V Serotonin localization in the turkey vaginal but not sperm storage tubule epithelia. Poult Sci 87:356-61 (2008). PubMed: 18212381

Dabadie H & Geffard M Identification of tryptamine and tryptamine-serotonin neurons in the rat dorsal raphe nuclei using specific antibodies. Synapse 14:178-83 (1993). PubMed: 7687387

See all 3 publication references for this product.


If you publish research using ab8882 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Serotonin antibody

Customer FAQs regarding Serotonin antibody

Protocols for Serotonin antibody

Price and availability of products related to Serotonin antibody

Serotonin antibody - related products: