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Products:Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab47726 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SerpinB3 antibody - Amino end mature SCCA-1 (ab47726)
All lanes : Anti-SerpinB3 antibody - Amino end mature SCCA-1 (ab47726) at 1/1000 dilution
Lane 1 : HFL-1 Cell lysate
Lane 2 : HFL-1 Cell lysate
Lane 3 : HFL-1 Cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 45 kDa
Observed band size : 38,52 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-SerpinB3 antibody - Amino end mature SCCA-1(ab47726)
ICC/IF image of ab47726 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47726, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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