SerpinB3 antibody - Amino end mature SCCA-1 (ab47726)

Product Name 

SerpinB3 antibody - Amino end mature SCCA-1

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SerpinB3 - Amino end mature SCCA-1

Immunogen 

A synthetic peptide based on the aminoterminal end of mature human SerpinB3.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific for SerpinB3 and SerpinB4.

We have a range of domain specific antibodies for this target. For a full list please see all SerpinB3 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 45 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic: Seems to also be secreted in plasma by cancerous cells but at a low level

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs

Relevance 

SerpinB3 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered as a protein expressed by cervical squamous carcinoma cells. The protein was identified in two forms, with a pI range of 6.3-6.6, and a more acidic form with pI 5.9-6.2. When the locus for serpins on chromosome 18 was analyzed further, two genes were found between the loci for maspin (serpinB5) and PAI-2 (serpinB2), and the two gene products were identified as tandem repeats that had 92% identity.
These two genes coded for SCCA1 and SCCA2 (also named leupin by another group). The SCCA1 gene product is serpinB3, and the SCCA2 is serpinB4. Although the two gene products are 92% identical, the differences are thought to confer significant specificity differences to the two proteins. In addition, splice variants have been reported for both genes, leading to more heterogeneity.
SerpinB3 inhibits the papain like cysteine proteinases cathepsin L, cathepsin K and cathepsin S, while SCCA2 inhibits the chymotrypsin like serine proteinases cathepsin G and human mast cell chymase. Although the structure of SCCA1 is very similar to SCCA2, critical differences are the slightly different scissile bond in the pseudosubstrate, and the number of cysteines.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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