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Products:Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Overview
Product name
Anti-SerpinB3 antibody - Amino end mature SCCA-1
See all SerpinB3 products (3) ...
Description
Rabbit polyclonal to SerpinB3 - Amino end mature SCCA-1
Specificity
This antibody is specific for SerpinB3 and SerpinB4.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide based on the aminoterminal end of mature human SerpinB3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 45 kDa.
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
May act as a protease inhibitor to modulate the host immune response against tumor cells.
Tissue specificity
Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
Developmental stage
Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.
Cellular localization
Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level.
Target information above from: UniProt accessionP29508
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Protein T4 A antibody
- Protein T4-A antibody
- SCCA-1 antibody
see all
Database links
- Entrez Gene: 6317 Human
- Omim: 600517 Human
- SwissProt: P29508 Human
- SwissProt: Q8IXI3 Human
- Unigene: 227948 Human
Anti-SerpinB3 antibody - Amino end mature SCCA-1 images:
Western blot - SerpinB3 antibody - Amino end mature SCCA-1 (ab47726)
All lanes : Anti-SerpinB3 antibody - Amino end mature SCCA-1 (ab47726) at 1/1000 dilution
Lane 1 : HFL-1 Cell lysate
Lane 2 : HFL-1 Cell lysate
Lane 3 : HFL-1 Cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 45 kDa
Observed band size : 38,52 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-SerpinB3 antibody - Amino end mature SCCA-1(ab47726)
ICC/IF image of ab47726 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47726, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-SerpinB3 antibody - Amino end mature SCCA-1 (ab47726)
ab47726 has not yet been referenced specifically in any publications.
Publishing research using ab47726? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"