SerpinB3 antibody (ab55733)

Product Name 

SerpinB3 antibody

Product type 

Primary antibodies

Description 

Mouse monoclonal to SerpinB3

Immunogen 

Recombinant fragment, corresponding to amino acids 276-391 of Human SerpinB3

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic: Seems to also be secreted in plasma by cancerous cells but at a low level

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs

Relevance 

SerpinB3 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered as a protein expressed by cervical squamous carcinoma cells. The protein was identified in two forms, with a pI range of 6.3-6.6, and a more acidic form with pI 5.9-6.2. When the locus for serpins on chromosome 18 was analyzed further, two genes were found between the loci for maspin (serpinB5) and PAI-2 (serpinB2), and the two gene products were identified as tandem repeats that had 92% identity.
These two genes coded for SCCA1 and SCCA2 (also named leupin by another group). The SCCA1 gene product is serpinB3, and the SCCA2 is serpinB4. Although the two gene products are 92% identical, the differences are thought to confer significant specificity differences to the two proteins. In addition, splice variants have been reported for both genes, leading to more heterogeneity.
SerpinB3 inhibits the papain like cysteine proteinases cathepsin L, cathepsin K and cathepsin S, while SCCA2 inhibits the chymotrypsin like serine proteinases cathepsin G and human mast cell chymase. Although the structure of SCCA1 is very similar to SCCA2, critical differences are the slightly different scissile bond in the pseudosubstrate, and the number of cysteines.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

SerpinB3 antibody images:

References for SerpinB3 antibody (ab55733)

  This product has been used in:

Burgener A et al. Identification of differentially expressed proteins in the cervical mucosa of HIV-1-resistant sex workers. J Proteome Res 7:4446-54 (2008). WB; Human. PubMed: 18707157

If you publish research using ab55733 please let us know so that we can cite the reference on this datasheet.

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