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Product Name
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SerpinB8 antibody [PI-8]
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See all SerpinB8 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PI-8] to SerpinB8
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Immunogen
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Recombinant full length protein (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 1.5 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Platelets
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Relevance
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SerpinB8 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in the placenta in a search for serpins similar to serpinB6. Chondrocyte like cells were shown to have enhanced expression of SerpinB8 after tumor necrosis factor alpha stimulation. Megakaryocytes have been shown to synthesize SerpinB8 and the protein is stored in platelets prior to release. SerpinB8 has also been shown to be elevated in the remodeling kidney, and message has been detected in normal human spleen, pituitary gland, epidermis, lung, liver, stomach, pancreas, and the mucosa oropharynx.
SerpinB8 has been shown to inhibit trypsin and thrombin. In addition to the RCL cleavage site, SerpinB8 has two paired dibasic consensus sites which flank the RCL cleavage sites for trypsin, chymotrypsin and thrombin, and allow it to inhibit furin.
The original sequence described was 374 amino acids in length, with predicted mass of 42.79 kDa and pI of 5.28. A 242 amino acid long form that terminates before the RCL, and thus would be inactive as a proteinase inhibitor, has a predicted mass of 27.7 kDa and a pI of 4.61. The relative abundance or distribution of the smaller form is unknown.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PI-8
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 0.5M Sodium chloride, pH 7.5
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Form
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Liquid
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Concentration
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0.820 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SerpinB8 antibody [PI-8] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for SerpinB8 antibody [PI-8] (ab49634)
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This product has been used in: (two most recent references)
Leblond J et al. The serpin proteinase inhibitor 8: an endogenous furin inhibitor released from human platelets. Thromb Haemost 95:243-52 (2006). PubMed: 16493485
Strik MC et al. Distribution of the human intracellular serpin protease inhibitor 8 in human tissues. J Histochem Cytochem 50:1443-54 (2002). PubMed: 12417609
If you publish research using ab49634 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SerpinB8
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