|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SerpinB9 antibody - Amino end mature Serpin-B9(ab47734)
(enlarge)
|
|
|
|
|
Product Name
|
SerpinB9 antibody - Amino end mature Serpin-B9
|
| |
See all SerpinB9 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to SerpinB9 - Amino end mature Serpin-B9
|
|
Immunogen
|
A synthetic peptide based on the aminoterminal end of mature human SerpinB9
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
This antibody is specific for SerpinB9.
We have a range of domain specific antibodies for this target. For a full list please see all SerpinB9 antibodies
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at an assay dependent dilution.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 43 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Immunology >> Immune Interventions >> Transplantation
|
|
Relevance
|
SerpinB9 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. The mouse orthologue to PI9 is SPI-6. SerpinB9 was first discovered in human bone marrow in a search for serpins similar to SerpinB6. SerpinB9 was identified in lymphocytes, especially natural killer cells and cytotoxic T lymphocytes, cells which also produce and store the apoptosis-inducing enzyme Granzyme B. PI9 was shown to be a potent inhibitor of Granzyme B, working at picamolar efficiencies. PI9 was also shown to inhibit caspase 1, the interleukin 1 converting enzyme, thus blocking IL1ß production, and decreasing inflammatory processes. Most leukemia cells and many tumor cells produce PI9, leading to speculation that PI9 may help protect tumor cells from destruction by NK and CTL cells. PI9 is also found in placenta, lung, kidney, mast cells, and many tissues and cell lines. In kidney transplant, PI9 is sharply elevated, and in the liver PI9 has been shown to be upregulated by a downstream estrogen promoter. SerpinB9 inhibits Granzyme B, and is cleaved by Granzyme M, as a down regulatory step. SerpinB9 also inhibits the bacterial proteinase subtilisin A, and this may be a protective agent against infections. Neutrophil elastase is also a target of SerpinB9. Like SerpinB6 and SerpinB8, SerpinB9 lacks a signal sequence, and is found mainly in the cytoplasm and nucleus, although it can be detected outside of cells and in serum. The original SerpinB9 sequence described was 379 amino acids in length, with predicted mass of 42.4 kDa and pI of 5.51.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SerpinB9 antibody - Amino end mature Serpin-B9 is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for SerpinB9 antibody - Amino end mature Serpin-B9 images, references, products related to ab47734 and other tools.
|
| |
SerpinB9 antibody - Amino end mature Serpin-B9 images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SerpinB9 antibody - Amino end mature Serpin-B9(ab47734)
Ab47734 staining human normal placenta tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
|
| |
| |
|
Search PubMed (MEDLINE) for references to SerpinB9
|
| |
SerpinB9 antibody - Amino end mature Serpin-B9 - more information
|
Customer reviews (feedback) regarding SerpinB9 antibody - Amino end mature Serpin-B9 |
Customer FAQs regarding SerpinB9 antibody - Amino end mature Serpin-B9 |
Protocols for SerpinB9 antibody - Amino end mature Serpin-B9 |
Price and availability of products related to SerpinB9 antibody - Amino end mature Serpin-B9 |
| |
SerpinB9 antibody - Amino end mature Serpin-B9 - related products:
|
|
|
| |
SerpinB9 antibody - Amino end mature Serpin-B9 - other tools:
|
Contact Abcam with a Technical Enquiry about SerpinB9 antibody - Amino end mature Serpin-B9 |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
