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Products:Immunology >> Immune Interventions >> Transplantation
Overview
Product name
Anti-SerpinB9 antibody - Amino end mature Serpin-B9
See all SerpinB9 products (8) ...
Description
Rabbit polyclonal to SerpinB9 - Amino end mature Serpin-B9
Specificity
This antibody is specific for SerpinB9.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide based on the aminoterminal end of mature human SerpinB9
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Immunology >> Immune Interventions >> Transplantation
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SerpinB9 antibody - Amino end mature Serpin-B9(ab47734)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab47734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
WB
Application notes
IHC-P: Use at an assay dependent dilution.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 43 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Granzyme B inhibitor.
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP50453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAP 3 antibody
- CAP-3 antibody
- CAP3 antibody
see all
Database links
- Entrez Gene: 5272 Human
- Omim: 601799 Human
- SwissProt: P50453 Human
- Unigene: 104879 Human
Anti-SerpinB9 antibody - Amino end mature Serpin-B9 images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SerpinB9 antibody - Amino end mature Serpin-B9(ab47734)
Ab47734 staining human normal placenta tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-SerpinB9 antibody - Amino end mature Serpin-B9 (ab47734)
ab47734 has not yet been referenced specifically in any publications.
Publishing research using ab47734? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"