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Anti-SerpinB9 antibody [PI9-17] (ab36624)

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Overview

Product name

Anti-SerpinB9 antibody [PI9-17]
See all SerpinB9 products (8) ...

Description

Mouse monoclonal [PI9-17] to SerpinB9

Specificity

Ab36624 recognises Proteinase Inhibitor 9, as well as Proteinase Inhibitor 9 complexed to granzyme B. It shows no cross reactivity with other homologous serpins (PI6, PI8 and PAI2) was observed.

Tested applications

IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length human protein produced in Escherichia coli.

Positive control

WB: YT-Indy cells (a natural killer leukemia cell line).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, serum free culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

PI9-17

Isotype

IgG1

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors
Immunology >> Immune Interventions >> Transplantation

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P

     

  • WB

     

Application notes

IHC-P: 1/100. Recommeneded pretreatment with 10 mM citric acid pH 6.0 for 20 min. For fixed cytospin preparations fix cells for 10 min in 10% formalin and then treat them as paraffin sections.

WB: Use at an assay dependent dilution. Detects a band of approximately 42, 67 kDa.

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Granzyme B inhibitor.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP50453 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CAP 3 antibody
  • CAP-3 antibody
  • CAP3 antibody
  • Cytoplasmic antiproteinase 3 antibody
  • Peptidase inhibitor 9 antibody
  • PI-9 antibody
  • PI9 antibody
  • Protease inhibitor 9 antibody
  • Protease inhibitor 9 (ovalbumin type) antibody
  • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 9 antibody
  • Serpin B9 antibody
  • Serpin peptidase inhibitor clade B (ovalbumin) member 9 antibody
  • Serpin peptidase inhibitor clade B member 9 antibody
  • SERPINB9 antibody
  • SPB9_HUMAN antibody
see all

Database links

References for Anti-SerpinB9 antibody [PI9-17] (ab36624)

ab36624 has not yet been referenced specifically in any publications.

Publishing research using ab36624? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"