SerpinB9 antibody [PI9-17] (ab36624)

Product Name 

SerpinB9 antibody [PI9-17]

Product type 

Primary antibodies

Description 

Mouse monoclonal [PI9-17] to SerpinB9

Immunogen 

Recombinant full length human protein produced in Escherichia coli.

Reacts with 
(species key)

Hu

Specificity 

Ab36624 recognises Proteinase Inhibitor 9, as well as Proteinase Inhibitor 9 complexed to granzyme B. It shows no cross reactivity with other homologous serpins (PI6, PI8 and PAI2) was observed.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100. Recommeneded pretreatment with 10 mM citric acid pH 6.0 for 20 min. For fixed cytospin preparations fix cells for 10 min in 10% formalin and then treat them as paraffin sections.

WB: Use at an assay dependent dilution. Detects a band of approximately 42, 67 kDa.

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: YT-Indy cells (a natural killer leukemia cell line).

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs

Immunology >> Immune Interventions >> Transplantation

Relevance 

SerpinB9 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. The mouse orthologue to PI9 is SPI-6. SerpinB9 was first discovered in human bone marrow in a search for serpins similar to SerpinB6. SerpinB9 was identified in lymphocytes, especially natural killer cells and cytotoxic T lymphocytes, cells which also produce and store the apoptosis-inducing enzyme Granzyme B. PI9 was shown to be a potent inhibitor of Granzyme B, working at picamolar efficiencies. PI9 was also shown to inhibit caspase 1, the interleukin 1 converting enzyme, thus blocking IL1ß production, and decreasing inflammatory processes. Most leukemia cells and many tumor cells produce PI9, leading to speculation that PI9 may help protect tumor cells from destruction by NK and CTL cells. PI9 is also found in placenta, lung, kidney, mast cells, and many tissues and cell lines. In kidney transplant, PI9 is sharply elevated, and in the liver PI9 has been shown to be upregulated by a downstream estrogen promoter. SerpinB9 inhibits Granzyme B, and is cleaved by Granzyme M, as a down regulatory step. SerpinB9 also inhibits the bacterial proteinase subtilisin A, and this may be a protective agent against infections. Neutrophil elastase is also a target of SerpinB9. Like SerpinB6 and SerpinB8, SerpinB9 lacks a signal sequence, and is found mainly in the cytoplasm and nucleus, although it can be detected outside of cells and in serum. The original SerpinB9 sequence described was 379 amino acids in length, with predicted mass of 42.4 kDa and pI of 5.51.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

PI9-17

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, serum free culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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