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Overview

  • Product nameSerum Amyloid A Human ELISA KitSee all Serum Amyloid A kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.5 ng/ml
  • Range
    0.41 ng/ml - 300 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.75 86% - 102%
    Serum 114.8 105% - 124%
    Plasma 112.1 83% - 137%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100635 Serum Amyloid A Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Serum Amyloid A in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human Serum Amyloid A coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum Amyloid A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Serum Amyloid A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Serum Amyloid A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceThe serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.
  • Cellular localizationSecreted
    • Alternative names
        Amyloid fibril protein AAAmyloid protein AMGC111216
        OTTMUSP00000045357PIG4SAASAA1Serum amyloid A 1Serum amyloid A 1 proteinserum amyloid A proteinSerum amyloid A protein precursor Serum amyloid A1 isoform 1 Serum amyloid A1 isoform 2TP53I4Tumor protein p53 inducible protein 4
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100635 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Serum Amyloid A Human ELISA Kit images

      • ELISA - Serum Amyloid A Human ELISA Kit (ab100635)
        ELISA - Serum Amyloid A Human ELISA Kit (ab100635)

      Protocols

      References for Serum Amyloid A Human ELISA Kit (ab100635)

      ab100635 has not yet been referenced specifically in any publications.

      Publishing research using ab100635 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100635 Submit a Question for ab100635

      Displaying 1 - 7 of 7 results for Abreviews and Q&A

      10
      Votes: Highest First

      ab100635: datasheet shows this antibody has been used in tissue culture supernatant, serum and plasma but has this been tested in Urine. Is there any data to show that and if possible any references where this kit has been used in urine.

      we have not tested this kit with urine, Please note that this does not necessarily exclude the use of urine (or any other body fluid for that matter) with this kit,

      Keep in mind that our ELISA kits are very accommodating and I see no reason why u...

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      I am following up on our conversation today about the SAA ELISA I recently ran. We did not dilute the samples at all. We used 100ul of serum. Attached are the results of our ELISA with a linear standard curve. The R2 is poor at 0.874.

      Thank you for your patience whilst I talked to the lab about your results. After reviewing it, there are a few points and suggestions we can give which are summarized below

      You could try a 2-fold sample dilution for your serum samples (range is ...

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      Inquiry: We have characterized our serum samples using your ELSIA kit ab100635 (Serum Amyloid A – human). Moving forward, we want to port this assay to our novel instrumentation for multiplexing capabilities. I see in your scientific suppo...

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      Thank you for your inquiry. Unfortunately we are unable to sell these antibodies apart from the kit. Please let me know if I can be of further assistance.

      How much serum should be used per well in this kit?

      Thank you for contacting Abcam regarding ab100635.


      Regarding sample volume, please use 100ul of serum. Your sample may require further dilution, but due to variations in sample, we recommend the dilution be determined by the end user.
      Read More

      Dear,



      I bought a Serum Amyloid A Human ELISA Kit (ab100635) from abcam for testing theSAA concentration in serum andin HDL. I notice that the standard curve you have is in ng/ml. I am wondering how much fold of dilution if I am test...

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      Thank you for contacting us.


      Our suggested dilution fold for SAA of normal human serum is 2 – 10X. Please note that the levels of the SAA may vary between different specimens. Optimal dilution factors for each sample must be determin...

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      Sir,

      Could you please let me know the dilution (if any) of normal human serum when using the following Abcam ELISAs:

      1. Serum Amyloid A (ab100635)
      2. IL2 receptor alpha (ab48885)

      I appreciate that serum levels of some...

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      Thank you for contacting us.

      Please find below therecommendations we obtained from the sources of the kits :

      ab100635 : our suggested dilution fold for SAA of normal human serum is 2 – 10X. Please note that the levels of the SAA...

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      Will this kit detect feline serum amyloid A?

      Thank you for your inquiry.  I was in contact with the lab and they do not currently have any cross-reactivity data on feline with ab100635.  If it helps though, we do know that both the capture and detection antibodies are monoclonal and that the...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"