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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serum Amyloid A antibody [115] (ab687)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serum Amyloid A antibody [115] (ab687)](\ps\datasheet\images\0\ab687\Serum-Amyloid-A-Primary-antibodies-ab687-2.jpg)
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Product Name
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Serum Amyloid A antibody [115]
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See all Serum Amyloid A antibodies (15)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [115] to Serum Amyloid A
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Immunogen
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Highly purified recombinant human serum amyloid A (MW: 12 kDa).
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with natural and recombinant human Serum Amyloid A (SAA) and does not cross-react with other human cytokines or growth factors tested such as IL-1beta, IL-8, MCAF, TGF-beta and EGF.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ProteinChip: Use at an assay dependent concentration.
This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Expressed by the liver; secreted in plasma.
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Research areas
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Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Lipoproteins
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Blood >> Acute Phase Reactants
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Relevance
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The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP.
SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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115
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Isotype
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IgG1
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Light chain type
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kappa
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Storage buffer
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Preservative: None
Constituents: PBS
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Purification notes
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Affinity purified from ascitic fluid.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Serum Amyloid A antibody [115] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Serum Amyloid A antibody [115] images, references, products related to ab687 and other tools.
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Serum Amyloid A antibody [115] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serum Amyloid A antibody [115] (ab687)
Ab687 staining human normal liver. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Serum Amyloid A antibody [115] (ab687)
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This product has been used in: (two most recent references)
Christenson K et al. Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7-sensitive pathway independent of formyl peptide receptor-like 1. J Leukoc Biol 83:139-48 (2008). PubMed: 17962368
Rossi L et al. Inflammatory protein profile during systemic high dose interleukin-2 administration. Proteomics 6:709-20 (2006). PubMed: 16342236
If you publish research using ab687 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Serum Amyloid A
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Serum Amyloid A antibody [115] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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