Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295)

Overview

• Product nameAnti-Serum Response Factor SRF (phospho S77) antibodySee all Serum Response Factor SRF primary antibodies ...
• Description
  Rabbit polyclonal to Serum Response Factor SRF (phospho S77)
• Specificityab79295 detects endogenous levels of Serum Response Factor SFR only when phosphorylated at serine 77.
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 77 (LYS^PGS).

• Positive controlIHC: human brain tissue WB: extracts from Jurkat cells, treated with PMA

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab79295 was purified from rabbit antiserum by chromatography using an epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Mesoderm

  • Stem Cells
  • Lineage Markers
  • Mesoderm

  • Other Factors

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
• Sequence similaritiesContains 1 MADS-box domain.
• Post-translational
  modificationsPhosphorylated by PRKDC.
• Cellular localizationNucleus.

Target information above from: UniProt accession P11831 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6722 Human
  • Entrez Gene: 20807 Mouse
  • Omim: 600589 Human
  • SwissProt: P11831 Human
  • SwissProt: Q9JM73 Mouse
  • Unigene: 520140 Human
  • Unigene: 45044 Mouse

• Alternative names
  c fos serum response element binding factor antibodyc fos serum response element binding transcription factor antibodyELK3 antibody

  ERP antibodyMCM 1 antibodyMCM1 antibodyOTTHUMP00000039820 antibodySAP2 antibodySerum response factor antibodySRF antibodySRF serum response factor c fos serum response element binding transcription factor antibodySRF_HUMAN antibody

  see all

Anti-Serum Response Factor SRF (phospho S77) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Serum Response Factor SRF (phospho S77) antibody (ab79295)
  Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab79295, at 1/50 - 1/100 dilution, in the absence and presence of the immunising peptide.
• 

  Western blot - Serum Response Factor SRF (phospho S77) antibody (ab79295)
  All lanes : Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295) at 1/1000 dilution
  
  Lane 1 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins)
  Lane 2 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins) with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  Additional bands at : 110 kDa,120 kDa. We are unsure as to the identity of these extra bands.