Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295)

Overview

  • Product nameAnti-Serum Response Factor SRF (phospho S77) antibodySee all Serum Response Factor SRF primary antibodies ...
  • Description
    Rabbit polyclonal to Serum Response Factor SRF (phospho S77)
  • Specificityab79295 detects endogenous levels of Serum Response Factor SFR only when phosphorylated at serine 77.
  • Tested applicationsICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 77 (LYSPGS).

  • Positive control
    • IHC: human brain tissue WB: extracts from Jurkat cells, treated with PMA

Properties

Applications

Our Abpromise guarantee covers the use of ab79295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similaritiesContains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF (phospho S77) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab79295, at 1/50 - 1/100 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295) at 1/1000 dilution

    Lane 1 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins)
    Lane 2 : extracts from Jurkat cells, treated with PMA (125 ng/ml, 30 mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 110 kDa,120 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Serum Response Factor SRF (phospho S77) antibody (ab79295)

ab79295 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.75% · Temperature: 4°C
Sample Pig Cell (Pig Nucleus Pulposus Cells)
Specification Pig Nucleus Pulposus Cells
Permeabilization Yes - TritonX
Fixative Paraformaldehyde
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Verified customer

Submitted Sep 26 2013

Thank you for your reply.

Do you have a positive control sample available for testing with this antibody? This antibody will only detect SFR when phosphorylated on S77. This modification may not be present in your fibroblast sample. The posit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"