Sheep IgG secondary antibody - H&L (ab6743)

Product Name 

Sheep IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Sheep IgG - H&L (FITC)

Immunogen 

Sheep whole molecule

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Sheep IgG secondary antibody - H&L (ab6743)

  This product has been used in: (two most recent references)

Chiriva-Internati M et al. AKAP-4: a novel cancer testis antigen for multiple myeloma. Br J Haematol 140:465-8 (2008). PubMed: 18217892

Shah MD et al. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets 19:365-72 (2008). PubMed: 18791943
If you publish research using ab6743 please let us know so that we can cite the reference on this datasheet.

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