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Read our guarantee »Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Rabbit anti-Sheep IgG H&L (TR) secondary antibody
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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TR)
Sheep
Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
Sheep IgG whole molecule
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
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IgG fraction
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Polyclonal
Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> Texas Red
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TR) (ab6745)
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Our Abpromise guarantee covers the use of ab6745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent concentration.
Flow Cyt: 1/500 - 1/2500.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
ICC/IF: 1/1000 - 1/5000.(PubMed: 17895403)
ELISA: 1/10000 - 1/50000.
Immunocytochemistry - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TR) (ab6745)

Ab6745 was used at dilution 1/100 with the primary antibody ab30524 in ICC/IF. See the review on ab30524.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab6745? Please let us know so that we can cite the reference in this datasheet
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Ab6745 was used at dilution 1/100 with the primary antibody ab30524 in ICC/IF. See the review on ab30524.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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