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Product Name
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Sheep IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Sheep IgG - H&L (TR)
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Immunogen
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Sheep IgG whole molecule
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution (PMID 17895403).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Cellular localization
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Secreted
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Research areas
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Secondary antibodies >> anti-Sheep >> IgG >> Fluorophore >> Texas Red
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
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Form
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Liquid
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Conjugation
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Texas Red ®
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Texas RedTM is a registered trademark of Molecular Probes Inc.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Sheep IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Sheep IgG secondary antibody - H&L (ab6745)
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This product has been used in: (two most recent references)
Jones S et al. Peripheral tachykinins and the neurokinin receptor NK1 are required for platelet thrombus formation. Blood 111:605-12 (2008). ICC/IF. PubMed: 17895403
Czaja K et al. Leptin receptors, NPY, and tyrosine hydroxylase in autonomic neurons supplying fat depots in a pig. Biochem Biophys Res Commun 293:1138-44 (2002). PubMed: 12051778
If you publish research using ab6745 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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