|
|
|
|
|
Product Name
|
Sheep IgG secondary antibody - H&L
|
|
Product type
|
Secondary antibodies
|
|
Description
|
Rabbit polyclonal to Sheep IgG - H&L (AP)
|
|
Immunogen
|
Sheep IgG whole molecule
|
|
Tested applications
(see key)
|
Dot, ELISA, IHC-Fr, IHC-P, IM, WB
|
|
Application notes
(see key)
|
Recommended dilutions
suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
|
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Secondary antibodies >> anti-Sheep >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
|
|
Relevance
|
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
|
|
|
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Purity
|
IgG fraction
|
|
Conjugation notes
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, 0.01% Thimerisol, pH 7.6 Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS
|
|
Purification notes
|
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
|
|
Form
|
Liquid
|
|
Conjugation
|
Alkaline Phosphatase
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C. Do not freeze.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Sheep IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for Sheep IgG secondary antibody - H&L (ab6748)
|
|
This product has been used in:
Corti D et al. Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individuals. PLoS One 5:e8805 (2010). ELISA; Human. PubMed: 20098712
If you publish research using ab6748 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IgG
|
| |
Sheep IgG secondary antibody - H&L - more information
|
Customer reviews (feedback) regarding Sheep IgG secondary antibody - H&L |
Customer FAQs regarding Sheep IgG secondary antibody - H&L |
Protocols for Sheep IgG secondary antibody - H&L |
Price and availability of products related to Sheep IgG secondary antibody - H&L |
| |
Sheep IgG secondary antibody - H&L - other tools:
|
Contact Abcam with a Technical Enquiry about Sheep IgG secondary antibody - H&L |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
