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Simian Virus 40 Major Capsid VP1 protein (ab74565)

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Overview

  • Product nameSimian Virus 40 Major Capsid VP1 protein
  • Protein descriptionRecombinant full length Simian Virus 40 Major Capsid VP1 protein expressed in S.cerevisiae, 44kDa. Swiss ID = P03087.
  • Expression hostSaccharomyces cerevisiae

    • Properties

  • Purification notesPurified by ultracentifugation. Shown to be more than 85% pure by SDS-PAGE.
  • FormLyophilised:Reconstitute with deionized H2O.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab74565 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA
    WB
    SDS-PAGE
  • Application notesELISA: Use at a concentration of 0.5 - 5 µg/ml to coat a microtitre plate..
    WB: Use at a concentration of 1 - 5 µg/lane. Predicted molecular weight: 40 kDa.
    SDS-PAGE: Use at a concentration of 4.2 µg/lane.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Major capsid protein VP1Major structural protein VP1
  • RelevanceSimian virus 40 (SV40) is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The simian virus 40 capsid is composed of 72 pentamers of VP1, the major protein of SV40. These pentamers are arranged in a T=7d icosahedral surface lattice, which is maintained by three types of appropriately arranged, non-equivalent interactions between the pentamers. Simian Virus 40 Major Capsid VP1 binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion enters a caveolae and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. The assembly takes place in the cell nucleus, encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release.
  • Cellular localizationVirion. Nucleus. Endoplasmic reticulum. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, viral DNA is translocated to the nucleus. Shortly after synthesis, a nuclear localization signal directs VP1 to the cell nucleus where virion assembly occurs.

    • Simian Virus 40 Major Capsid VP1 protein images

    • SDS-PAGE - Simian Virus 40 Major Capsid VP1 protein (ab74565)
      SDS-PAGE - Simian Virus 40 Major Capsid VP1 protein (ab74565)
      SDS-PAGE showing ab74565 at approximately 44kDa (4.2µg/lane)

    References for Simian Virus 40 Major Capsid VP1 protein (ab74565)

    ab74565 has not yet been referenced specifically in any publications.

    Publishing research using ab74565 ? Please let us know so that we can cite the reference in this datasheet

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    Can I also detect ab74565 (SV40 VP1) with this mAb ab34756 (anti-Hu JCV)? Please confirm it.

    Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this wi...

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    Are these proteins tagged?

    Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"