Recombinant Simian Virus 40 Simian Virus 40 Major Capsid VP1 protein (ab74565)
Key features and details
- Expression system: Saccharomyces cerevisiae
- Purity: > 85% SDS-PAGE
- Suitable for: ELISA, WB, SDS-PAGE
Description
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Product name
Recombinant Simian Virus 40 Simian Virus 40 Major Capsid VP1 protein -
Purity
> 85 % SDS-PAGE.
Purified by ultracentifugation -
Expression system
Saccharomyces cerevisiae -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Simian Virus 40 -
Sequence
MKMAPTKRKGSCPGAAPKKPKEPVQVPKLVIKGGIEVLGVKTGVDSFTEV ECFLNPQMGNPDEHQKGLSKSLAAEKQFTDDSPDKEQLPCYSVARIPLPN LNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHENGAGKPIQGS NFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKAVL DKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDE QGVGPLCKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKN PYPISFLLSDLINRRTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMI RYIDEFGQTTTRLQ
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Specifications
Our Abpromise guarantee covers the use of ab74565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituent: PBS
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ReconstitutionReconstitute with deionized H2O.
General Info
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Alternative names
- Major capsid protein VP1
- Major structural protein VP1
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Relevance
Simian virus 40 (SV40) is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The simian virus 40 capsid is composed of 72 pentamers of VP1, the major protein of SV40. These pentamers are arranged in a T=7d icosahedral surface lattice, which is maintained by three types of appropriately arranged, non-equivalent interactions between the pentamers. Simian Virus 40 Major Capsid VP1 binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion enters a caveolae and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. The assembly takes place in the cell nucleus, encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release. -
Cellular localization
Virion. Nucleus. Endoplasmic reticulum. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, viral DNA is translocated to the nucleus. Shortly after synthesis, a nuclear localization signal directs VP1 to the cell nucleus where virion assembly occurs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab74565 has not yet been referenced specifically in any publications.