Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab29330 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)

    ab29330 has not yet been referenced specifically in any publications.

    Product Wall


    I have heard back from the lab concerning donor information for the lots requested of human, mouse and rat skeletal tissue lysates.


    Skeletal Muscle (Human) Tissue Lysate: 24 year old normal male

    Skeletal Muscle (Rat) Tiss...

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    Thank you for contacting us again.
    Regarding this item - Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330), the donor tissue was normal and doesn’t have ACTN3 R577X mutation information available.
    I hope this was helpful. ...

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    Thank you for contacting us.
    Skeletal Muscle (Human) Tissue Lysate is recommended as a positive control for ab68204. Both products are recommended for Western Blotting, and are guaranteed to work in this application.
    If you had any problems u...

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    Hier nun die Antwort aus unserem Labor:

    "The lysis buffers are all the same for the lysates. The components of the lysis buffer is not intended to be disclosed due to company policy. The muscle tissues are mixed with all types...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"