Anti-Slit1 antibody (ab10984)
Key features and details
- Rabbit polyclonal to Slit1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Slit1 antibody
See all Slit1 primary antibodies -
Description
Rabbit polyclonal to Slit1 -
Host species
Rabbit -
Specificity
The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002). -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide:
CSAKEQYFIPGTEDYHLN
conjugated to KLH, corresponding to amino acids 487-504 of Mouse Slit1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa). |
Target
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Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. -
Tissue specificity
Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 20562 Mouse
- Entrez Gene: 65047 Rat
- SwissProt: Q80TR4 Mouse
- SwissProt: O88279 Rat
- Unigene: 40322 Mouse
- Unigene: 30002 Rat
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Alternative names
- KIAA0813 antibody
- MEGF 4 antibody
- MEGF4 antibody
see all
Images
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ab10984-Rabbit polyclonal to Slit1 (1/500)
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min
Lane 1: Rat Brain tissue lysate
Lane 2: Mouse Brain tissue LysateNote: The smaller strong bands observed in this WB are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab: Goat anti-rabbit HRP ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min Lane 1: Rat Brain tissue lysate Lane 2: Mouse Brain tissue Lysate Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of
Datasheets and documents
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Datasheet download
References (0)
ab10984 has not yet been referenced specifically in any publications.