Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
Key features and details
- Mouse monoclonal [NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain
- Suitable for: IHC-P, WB
- Reacts with: Rat, Rabbit
- Isotype: IgG1
Overview
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Product name
Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]
See all Slow Skeletal Myosin Heavy chain primary antibodies -
Description
Mouse monoclonal [NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Rabbit
Predicted to work with: Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Human Slow Skeletal Myosin Heavy chain. Human skeletal muscle myosin purified from myofibrils.
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Positive control
- IHC-P: Rabbit tongue tissue. WB: Rat skeletal muscle tissue extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR201056, GR231200, GR285981. Lot numbers other than GR201056, GR231200, GR285981will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
NOQ7.5.4D -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (5) |
Use a concentration of 2.5 - 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).
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Notes |
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IHC-P
Use a concentration of 2.5 - 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa). |
Target
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Function
Muscle contraction. -
Tissue specificity
Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level). -
Involvement in disease
Defects in MYH7 are the cause of cardiomyopathy familial hypertrophic type 1 (CMH1) [MIM:192600]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYH7 are the cause of myopathy myosin storage (MYOMS) [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers.
Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM) [MIM:181430]; also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm.
Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S) [MIM:613426]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in MYH7 are the cause of myopathy distal type 1 (MPD1) [MIM:160500]. MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 4625 Human
- Entrez Gene: 29557 Rat
- Omim: 160760 Human
- SwissProt: P12883 Human
- SwissProt: P02564 Rat
- Unigene: 719946 Human
- Unigene: 225886 Rat
- Unigene: 54399 Rat
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Alternative names
- Beta myosin heavy chain antibody
- cardiac muscle beta isoform antibody
- CMD1S antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rabbit tomgue tissue staining Skeletal Myosin Heavy chain using ab11083 at 2.5 μg/mL.
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Lane 1 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1.25 µg/ml
Lane 2 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 2.5 µg/ml
Lane 3 : Negative control antibody
All lanes : Rat skeletal muscle
Secondary
All lanes : Anti-Mouse IgG (Fac-specific)-peroxidase conjugate at 1/5000 dilution
Predicted band size: 200 kDa -
ab11083 at 1/500 dilution, staining Slow Skeletal Myosin Heavy chain in mouse tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
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ab11083 at 1/4000 dilution, staining Slow Skeletal Myosin Heavy chain in rabbit tongue tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (99)
ab11083 has been referenced in 99 publications.
- Wen W et al. Dietary lycopene supplementation improves meat quality, antioxidant capacity and skeletal muscle fiber type transformation in finishing pigs. Anim Nutr 8:256-264 (2022). PubMed: 34988307
- Cefis M et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
- Chi MY et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
- Song YT et al. Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model. Bioact Mater 14:443-455 (2022). PubMed: 35415280
- Schrötter S et al. The non-essential TSC complex component TBC1D7 restricts tissue mTORC1 signaling and brain and neuron growth. Cell Rep 39:110824 (2022). PubMed: 35584673