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Anti-Smac / Diablo antibody [Y12] (ab32023)

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Overview

Product name

Anti-Smac / Diablo antibody [Y12]
See all Smac / Diablo products (14) ...

Description

Rabbit monoclonal [Y12] to Smac / Diablo

Tested applications

WB, IHC-P, ICC, IP, Flow Cyt, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to C terminal residues human Smac/Diablo

Epitope

ab32023 reacts with an epitope located in the C terminal region of Smac / Diablo.

Positive control

Jurkat cell lysate and skin cancer tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

Y12

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Receptors >> Death Receptors

  • Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12] (ab32023)Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12] (ab32023) image (enlarge)

  • Western blot - Smac / Diablo antibody [Y12] (ab32023)Western blot - Smac / Diablo antibody [Y12] (ab32023) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).

Tissue specificity

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.

Domain

The mature N-terminus mediates interaction with XIAP.

Cellular localization

Mitochondrion. Released into the cytosol when cells undergo apoptosis.

Target information above from: UniProt accessionQ9NR28 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • 0610041G12Rik antibody
  • DBLOH_HUMAN antibody
  • DBOH antibody
  • DBOH antibody
  • diablo antibody
  • Diablo homolog (Drosophila) antibody
  • Diablo homolog (Drosophila) antibody
  • Diablo homolog antibody
  • Diablo homolog mitochondrial antibody
  • DIABLO S antibody
  • DIABLO S antibody
  • Direct IAP binding protein with low pI antibody
  • Direct IAP binding protein with low pI antibody
  • Direct IAP-binding protein with low pI antibody
  • FLJ10537 antibody
  • FLJ25049 antibody
  • Mitochondrial Smac protein antibody
  • Second mitochondria derived activator of caspase antibody
  • Second mitochondria derived activator of caspase antibody
  • Second mitochondria-derived activator of caspase antibody
  • SMAC 3 antibody
  • Smac antibody
  • Smac protein antibody
  • Smac protein antibody
  • SMAC3 antibody
  • SMAC3 antibody
see all

Database links

see all

Anti-Smac / Diablo antibody [Y12] images:

  Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12] (ab32023)

Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12] (ab32023)

ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by Immunohistochemistry, Paraffin embedded tissue

  Western blot - Smac / Diablo antibody [Y12] (ab32023)

Western blot - Smac / Diablo antibody [Y12] (ab32023)

Anti-Smac / Diablo antibody [Y12] (ab32023) at 1/1000 dilution + Jurkat cell lysate

Predicted band size : 27 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)

References for Anti-Smac / Diablo antibody [Y12] (ab32023)

This product has been referenced in:

  • Augello C  et al. Inhibitors of apoptosis proteins (IAPs) expression and their prognostic significance in hepatocellular carcinoma. BMC Cancer 9:125 (2009). ICC/IF; Human.Read more (PubMed: 19397802) »

See 1 publication for this product

Publishing research using ab32023? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"