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Product name
Anti-Smac / Diablo antibody
See all Smac / Diablo products (14) ...
Description
Rabbit polyclonal to Smac / Diablo
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Rabbit, Human
Predicted to work with
Cow, Dog, Pig
Immunogen
A region within synthetic peptide: LQVEEVHQLS RKAETKLAEA QIEELRQKTQ EEGEERAESE QEAYLRED, corresponding to C terminal amino acids 139-186 of Human Smac / Diablo
LQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESE QEAYLRED
Positive control
HepG2 cell lysate for WB. Paraffin embedded human kidney tissue for IHC.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was purified by affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/15000.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 27 kDa.
Suggested dilution in 5% skim milk/PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
Domain
The mature N-terminus mediates interaction with XIAP.
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis.
Target information above from: UniProt accessionQ9NR28
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
see all
Database links
- Entrez Gene: 56616 Human
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- Unigene: 169611 Human
Anti-Smac / Diablo antibody images:
Western blot - Smac antibody (ab23450)
Anti-Smac / Diablo antibody (ab23450) at 1 µg/ml + HepG2 lysate
Secondary
HRP conjugated anti-Rabbit IgG (1: 50,000-100,000)
Predicted band size : 27 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Smac antibody (ab23450)
ab23450 at 4-8 ug/ml staining human Smac in kidney cells by immunohistochemistry (Paraffin embedded tissue). Epithelial cells of renal tubule (indicated with arrows). Magnification 400x.
References for Anti-Smac / Diablo antibody (ab23450)
ab23450 has not yet been referenced specifically in any publications.
Publishing research using ab23450? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"