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- Proteins and Peptides
- Secondary antibodies
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Product name
Anti-Smac / Diablo antibody
See all Smac / Diablo products (14) ...
Description
Rabbit polyclonal to Smac / Diablo
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Peptide (SDEGADQEEEAYLRED)corresponding to amino acids 222 to 237 of murineSmac/DIABLO (2).
SDEGADQEEEAYLRED
Positive control
Mouse heart tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.
IHC-P: Use at an assay dependent dilution.
Target
Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
Domain
The mature N-terminus mediates interaction with XIAP.
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis.
Target information above from: UniProt accessionQ9NR28
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
see all
Database links
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- SwissProt: Q5RK17 Rat
- Unigene: 169611 Human
see all
Anti-Smac / Diablo antibody images:
Western blot - Smac antibody (ab8115)
All lanes : Anti-Smac / Diablo antibody (ab8115) at 1 µg/ml
Lane 1 : Mouse heart tissue lysate with absence of blocking peptide
Lane 2 : Mouse heart tissue lysate with presence of blocking peptide
Lane 3 : Rat heart tissue lysate with absence of blocking peptide
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Smac antibody (ab8115)
ab8115 at 2µg/ml staining of Smac/Diablo in mouse spleen tissue by IHC
References for Anti-Smac / Diablo antibody (ab8115)
This product has been referenced in:
- Heazell AE et al. Altered expression of regulators of caspase activity within trophoblast of normal pregnancies and pregnancies complicated by preeclampsia. Reprod Sci 15:1034-43 (2008). WB; Human.Read more (PubMed: 19088373) »
See 1 publication for this product
Publishing research using ab8115? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"