Anti-Smad1 antibody [913C1b] (ab53745)

Overview

  • Product nameAnti-Smad1 antibody [913C1b]
    See all Smad1 primary antibodies
  • Description
    Mouse monoclonal [913C1b] to Smad1
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Smad1

  • Positive control
    • WB: HEK293, F2408 and NIH3T3 whole cell lysates, FC: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Flow Cyt Use at an assay dependent dilution.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSP 1 antibody
    • BSP-1 antibody
    • BSP1 antibody
    • Dwarfin A antibody
    • DwfA antibody
    • hSMAD 1 antibody
    • hSMAD1 antibody
    • JV 41 antibody
    • JV4 1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH 1 antibody
    • MADH1 antibody
    • Madr 1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • Sma and Mad related protein 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • SMAD mothers against DPP homolog 1 Drosophila antibody
    • Smad1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • Spinal muscular atrophy distal with upper limb predominance antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 antibody [913C1b] images

  • Using 0.5µg Anti-SMAD1 antibody (ab53745) and 0.25µg Goat anti-Mouse secondary.
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

References for Anti-Smad1 antibody [913C1b] (ab53745)

ab53745 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Loading amount 40 µg
Specification Fibroblasts
Gel Running Conditions Reduced Denaturing (10% Bis-Tris with MOPS buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Herr Dr. Daniel Meier

Verified customer

Submitted Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"