Anti-Smad1 antibody [913C1b] (ab53745)

Overview

• Product nameAnti-Smad1 antibody [913C1b]See all Smad1 primary antibodies ...
• Description
  Mouse monoclonal [913C1b] to Smad1
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant Smad1

• Positive controlWB: HEK293, F2408 and NIH3T3 whole cell lysates, FC: HeLa cells

Properties

• FormLiquid
• Storage instructionsStore at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number913C1b
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
• Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
  Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Target information above from: UniProt accession Q15797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4086 Human
  • Omim: 601595 Human
  • SwissProt: Q15797 Human
  • Unigene: 604588 Human

• Alternative names
  BSP 1 antibodyBSP-1 antibodyBSP1 antibody

  Dwarfin A antibodyDwfA antibodyhSMAD 1 antibodyhSMAD1 antibodyJV 41 antibodyJV4 1 antibodyJV4-1 antibodyJV41 antibodyMAD homolog 1 antibodyMAD mothers against decapentaplegic homolog 1 antibodyMad related protein 1 antibodyMad-related protein 1 antibodyMADH 1 antibodyMADH1 antibodyMadr 1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySma and Mad related protein 1 antibodySMA- AND MAD-RELATED PROTEIN 1 antibodySMAD 1 antibodySMAD family member 1 antibodySMAD mothers against DPP homolog 1 antibodySMAD mothers against DPP homolog 1 Drosophila antibodySmad1 antibodySmad1 antibodySMAD1_HUMAN antibodySpinal muscular atrophy distal with upper limb predominance antibodyTGF beta signaling protein 1 antibodyTransforming growth factor beta signaling protein 1 antibodyTransforming growth factor-beta-signaling protein 1 antibody

  see all

Anti-Smad1 antibody [913C1b] images

• 

  Flow Cytometry - Smad1 antibody [913C1b] (ab53745)
  Using 0.5µg Anti-SMAD1 antibody (ab53745) and 0.25µg Goat anti-Mouse secondary.
• 

  Western blot - Smad1 antibody [913C1b] (ab53745)
  Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
  
  Secondary
  Mouse IgG antibody at 1/2500 dilution
  
  Predicted band size : 52 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - Smad1 antibody [913C1b] (ab53745)
  Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg
  
  Secondary
  Mouse IgG at 1/2500 dilution
  
  Predicted band size : 52 kDa
  Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - Smad1 antibody [913C1b] (ab53745)
  Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + F2408 whole cell lysate at 25 µg
  
  Secondary
  Mouse IgG at 1/2500 dilution
  
  Predicted band size : 52 kDa
  Observed band size : 62 kDa (why is the actual band size different from the predicted?)