Overview

  • Product nameAnti-Smad1 antibody [EP565Y]
    See all Smad1 primary antibodies
  • Description
    Rabbit monoclonal [EP565Y] to Smad1
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Smad1.

  • Positive control
    • ICC/If: HeLa cells. WB: HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Smad1 antibody (Alexa Fluor® 647) [EP565Y] (ab206580)

Properties

Applications

Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • Application notesIs unsuitable for Flow Cyt,IHC or IP.
  • Target

    • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similaritiesBelongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Anti-Smad1 antibody [EP565Y] images

    • Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded mouse skin tissues sections, labelling Smad1 with ab33902 at a concentration of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed with 10 mM Citrate buffer at pH 6.0. Blocking was done with 5% serum for 1 hour at 21°C. Secondary used was a Donkey anti-Rabbit Alexa Fluor® 647 conjugate, this has been pseudocolored to green. Counterstain used was DAPI against nuclear DNA.

      See Abreview

    • Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded human pancreatic tumor sections, labelling Smad1 with ab33902 at a concentration of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed with 10 mM Citrate buffer at pH 6.0. Blocking was done with 5% serum for 1 hour at 21°C. Secondary used was a Donkey anti-Rabbit Alexa Fluor® 647 conjugate. Counterstain used was DAPI against nuclear DNA.

      See Abreview

    • Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 52 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.

    References for Anti-Smad1 antibody [EP565Y] (ab33902)

    This product has been referenced in:
    • Sakaki-Yumoto M  et al. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem 288:18546-60 (2013). Read more (PubMed: 23649632) »
    • Wu T  et al. miR-30 family members negatively regulate osteoblast differentiation. J Biol Chem 287:7503-11 (2012). WB . Read more (PubMed: 22253433) »

    See all 7 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
    Permeabilization No
    Specification skin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Oct 29 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization No
    Specification pancreatic tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Oct 29 2015

    Application Western blot
    Sample Human Cell lysate - nuclear (Normal Human Keratinocyte)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Normal Human Keratinocyte
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"