• Product nameAnti-Smad1 antibody [EP565Y]See all Smad1 primary antibodies ...
  • Description
    Rabbit monoclonal [EP565Y] to Smad1
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

  • Immunogen

    A synthetic peptide corresponding to residues in human Smad1.

  • Positive control
    • ICC/If: HeLa cells. WB: HeLa cell lysate.
  • General notesProduced under U.S. Patent No. 5,675,063.



Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
  • Application notesICC/IF: 1/100 - 1/250.
    WB: 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).

    Is unsuitable for FACS, IHC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similaritiesBelongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Target information above from: UniProt accession Q15797 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • BSP-1 antibody
      • BSP1 antibody
      • Dwarfin A antibody
      • DwfA antibody
      • HsMAD1 antibody
      • JV4 1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • Madr 1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • SMAD mothers against DPP homolog 1 Drosophila antibody
      • Smad1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • Spinal muscular atrophy distal with upper limb predominance antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Anti-Smad1 antibody [EP565Y] images

    • Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 52 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.

    References for Anti-Smad1 antibody [EP565Y] (ab33902)

    This product has been referenced in:
    • Sakaki-Yumoto M  et al. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem 288:18546-60 (2013). Read more (PubMed: 23649632) »
    • Wu T  et al. miR-30 family members negatively regulate osteoblast differentiation. J Biol Chem 287:7503-11 (2012). WB . Read more (PubMed: 22253433) »

    See all 7 Publications for this product

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