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Anti-Smad1 antibody [EP565Y] (ab33902)

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Overview

Product name

Anti-Smad1 antibody [EP565Y]
See all Smad1 products (15) ...

Description

Rabbit monoclonal [EP565Y] to Smad1

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues in human Smad1.

Positive control

ICC/If: HeLa cells. WB: HeLa cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

EP565Y

Isotype

IgG

Research areas

Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs

  • Western blot - Smad1 antibody [EP435E] (ab33902)Western blot - Smad1 antibody [EP435E] (ab33902) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Smad1 antibody [EP435E] (ab33902)Immunocytochemistry/ Immunofluorescence - Smad1 antibody [EP435E] (ab33902) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/100 - 1/250.
WB: 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).

Is unsuitable for FACS, IHC or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

Tissue specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Sequence similarities

Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.

Post-translational
modifications

Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

Cellular localization

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Target information above from: UniProt accessionQ15797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BSP-1 antibody
  • BSP1 antibody
  • Dwarfin A antibody
  • DwfA antibody
  • HsMAD1 antibody
  • JV4 1 antibody
  • JV4-1 antibody
  • JV41 antibody
  • MAD homolog 1 antibody
  • MAD mothers against decapentaplegic homolog 1 antibody
  • Mad related protein 1 antibody
  • Mad-related protein 1 antibody
  • MADH1 antibody
  • Madr 1 antibody
  • MADR1 antibody
  • Mothers against decapentaplegic homolog 1 antibody
  • Mothers against DPP homolog 1 antibody
  • SMAD 1 antibody
  • SMAD family member 1 antibody
  • SMAD mothers against DPP homolog 1 antibody
  • SMAD mothers against DPP homolog 1 Drosophila antibody
  • Smad1 antibody
  • Smad1 antibody
  • SMAD1_HUMAN antibody
  • Spinal muscular atrophy distal with upper limb predominance antibody
  • TGF beta signaling protein 1 antibody
  • Transforming growth factor beta signaling protein 1 antibody
  • Transforming growth factor-beta-signaling protein 1 antibody
see all

Database links

Anti-Smad1 antibody [EP565Y] images:

  Western blot - Smad1 antibody [EP435E] (ab33902)

Western blot - Smad1 antibody [EP435E] (ab33902)

Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate

Predicted band size : 52 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - Smad1 antibody [EP435E] (ab33902)

Immunocytochemistry/ Immunofluorescence - Smad1 antibody [EP435E] (ab33902)

Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.

References for Anti-Smad1 antibody [EP565Y] (ab33902)

This product has been referenced in:

  • Yanagisawa M  et al. Community effect triggers terminal differentiation of myogenic cells derived from muscle satellite cells by quenching Smad signaling. Exp Cell Res 317:221-33 (2011). WB; Mouse.Read more (PubMed: 20965167) »
  • Zhang L  et al. Modulation of TGF-{beta} signaling by endoglin in murine hemangioblast development and primitive hematopoiesis. Blood 118:88-97 (2011).Read more (PubMed: 21602526) »

See all 5 publications for this product

Publishing research using ab33902? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"