Overview

  • Product nameAnti-Smad1 antibodySee all Smad1 primary antibodies ...
  • Description
    Rabbit polyclonal to Smad1
  • SpecificityThis antibody recognizes ~60 kDa human Smad1.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) corresponding to amino acid residues around 253 of human Smad1.

Properties

Applications

Our Abpromise guarantee covers the use of ab18889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Target information above from: UniProt accession Q15797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BSP-1 antibody
    • BSP1 antibody
    • Dwarfin A antibody
    • DwfA antibody
    • HsMAD1 antibody
    • JV4 1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • Madr 1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • SMAD mothers against DPP homolog 1 Drosophila antibody
    • Smad1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • Spinal muscular atrophy distal with upper limb predominance antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

References for Anti-Smad1 antibody (ab18889)

This product has been referenced in:
  • Pannu J  et al. Transforming growth factor-beta receptor type I-dependent fibrogenic gene program is mediated via activation of Smad1 and ERK1/2 pathways. J Biol Chem 282:10405-13 (2007). WB ; Human . Read more (PubMed: 17317656) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Aortic smooth muscle)
Loading amount 20 µg
Specification Aortic smooth muscle
Gel Running Conditions Reduced Denaturing (10% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Christel van Erp

Verified customer

Submitted Mar 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"