Overview
- Product nameAnti-Smad1 antibodySee all Smad1 primary antibodies ...
- DescriptionRabbit polyclonal to Smad1
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Horse, Chicken, Cow, Human, Pig, Xenopus laevis, Chimpanzee, Gorilla - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad1.
(Peptide available as ab66722.)
- Positive controlThis antibody gave a positive signal in Mouse Heart Lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab46688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).Can be blocked with Smad1 peptide (ab66722). |
Target
- FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
- Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
- Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. - Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. - Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
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Database links
- Entrez Gene: 395680 Chicken
- Entrez Gene: 540488 Cow
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 397016 Pig
- Entrez Gene: 25671 Rat
- Entrez Gene: 443229 Sheep
- Entrez Gene: 399457 Xenopus laevis
- Omim: 601595 Human
- SwissProt: Q1JQA2 Cow
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- SwissProt: P97588 Rat
- Unigene: 604588 Human
- Unigene: 223717 Mouse
- Unigene: 10635 Rat
see all
Target information above from: UniProt accession
Q15797
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BSP-1 antibodyBSP1 antibodyHsMAD1 antibody
- JV4-1 antibodyJV41 antibodyMAD homolog 1 antibodyMAD mothers against decapentaplegic homolog 1 antibodyMad related protein 1 antibodyMad-related protein 1 antibodyMADH1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySMA- AND MAD-RELATED PROTEIN 1 antibodySMAD 1 antibodySMAD family member 1 antibodySMAD mothers against DPP homolog 1 antibodySmad1 antibodySmad1 antibodySMAD1_HUMAN antibodyTGF beta signaling protein 1 antibodyTransforming growth factor-beta-signaling protein 1 antibody
see all
Anti-Smad1 antibody images
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Western blot - Anti-Smad1 antibody (ab46688)Anti-Smad1 antibody (ab46688) at 1 µg/ml + Heart (Mouse) Tissue Lysate (ab27255) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes -
Western blotAnti-Smad1 antibody (ab46688) at 1 µg/ml +Smad1 protein (Tagged) (ab84653) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-Smad1 antibody (ab46688)
ab46688 has not yet been referenced specifically in any publications.
