Overview

  • Product nameAnti-Smad1 antibody
    See all Smad1 primary antibodies
  • Description
    Rabbit polyclonal to Smad1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Horse, Chicken, Cow, Human, Pig, Xenopus laevis, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad1.

    (Peptide available as ab66722.)

  • Positive control
    • This antibody gave a positive signal in Mouse Heart Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab46688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human Smad1 peptide (ab66722).

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 antibody images

  • Anti-Smad1 antibody (ab46688) at 1 µg/ml + Heart (Mouse) Tissue Lysate (ab27255) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Anti-Smad1 antibody (ab46688) at 1 µg/ml + Human Smad1 full length protein (ab84653) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Anti-Smad1 antibody (ab46688)

ab46688 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (HEK293 Ms Heart Ms Skeletal Muscle)
Loading amount 20 µg
Specification HEK293 Ms Heart Ms Skeletal Muscle
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 8-16%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 25 2010

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